The Effect of Elasticity of Gelatin Nanoparticles on the Interaction with Macrophages

Basit Öğe Kaydı
dc.authoridSchneider, Marc/0000-0002-9260-7357
dc.authoridWeiss, Agnes-Valencia/0000-0003-4767-7150
dc.contributor.authorYildirim, Metin
dc.contributor.authorWeiss, Agnes-Valencia
dc.contributor.authorSchneider, Marc
dc.date.accessioned2025-03-17T12:25:13Z
dc.date.available2025-03-17T12:25:13Z
dc.date.issued2023
dc.departmentTarsus Üniversitesi
dc.description.abstractGelatin is a biocompatible, biodegradable, cheap, and nontoxic material, which is already used for pharmaceutical applications. Nanoparticles from gelatin (GNPs) are considered a promising delivery system for hydrophilic and macromolecular drugs. Mechanical properties of particles are recognized as an important parameter affecting drug carrier interaction with biological systems. GNPs offer the preparation of particles with different stiffness. GNPs were loaded with Fluorescein isothiocyanate-labeled 150 kDa dextran (FITC-dextran) yielding also different elastic properties. GNPs were visualized using atomic force microscopy (AFM), and force-distance curves from the center of the particles were evaluated for Young's modulus calculation. The prepared GNPs have Young's moduli from 4.12 MPa for soft to 9.8 MPa for stiff particles. Furthermore, cytokine release (IL-6 and TNF-alpha), cell viability, and cell uptake were determined on macrophage cell lines from mouse (RAW 264.7) and human (dTHP-1 cells, differentiated human monocytic THP-1 cells) origin for soft and stiff GNPs. Both particle types showed good cell compatibility and did not induce IL-6 and TNF-alpha release from RAW 264.7 and dTHP-1 cells. Stiffer GNPs were internalized into cells faster and to a larger extent.
dc.identifier.doi10.3390/pharmaceutics15010199
dc.identifier.issn1999-4923
dc.identifier.issue1
dc.identifier.pmid36678828
dc.identifier.scopus2-s2.0-85146748079
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/pharmaceutics15010199
dc.identifier.urihttps://hdl.handle.net/20.500.13099/1564
dc.identifier.volume15
dc.identifier.wosWOS:000927119900001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofPharmaceutics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250316
dc.subjectnanotechnology
dc.subjectdrug delivery
dc.subjectatomic force microscopy
dc.subjectyoung's modulus
dc.subjectphagocytosis
dc.subjectcell uptake
dc.titleThe Effect of Elasticity of Gelatin Nanoparticles on the Interaction with Macrophages
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu

We collect and process your personal information for the following purposes: Authentication, Preferences, Acknowledgement and Statistics.
To learn more, please read our privacy policy.

Customize