Detection of Kallikrein-Related Peptidase 4 with a Label-free Electrochemical Impedance Biosensor Based on a Zinc(II) Phthalocyanine Tetracarboxylic Acid-Functionalized Disposable Indium Tin Oxide Electrode

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dc.authoridYUZER, ABDULCELIL/0000-0002-2287-4126
dc.authoridAYDIN, Elif Burcu/0000-0002-1982-4128
dc.authoridInce, Mine/0000-0002-9164-0446
dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorAydin, Muhammet
dc.contributor.authorYuzer, Abdulcelil
dc.contributor.authorInce, Mine
dc.contributor.authorOcakoglu, Kasim
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2025-03-17T12:25:54Z
dc.date.available2025-03-17T12:25:54Z
dc.date.issued2021
dc.departmentTarsus Üniversitesi
dc.description.abstractA new impedimetric biosensing system based on kallikrein-related peptidase 4 (KLK 4) antigen-specific antibodies and a zinc(II) phthalocyanine tetracarboxylic acid (Zn-PcTCa) matrix material was developed for the first time in this study. First, a Zn-PcTCa-coated indium tin oxide surface was used as an interface matrix material for the immobilization of anti-KLK 4 antibodies, and they bound to the platform via amide bonds. In the presence of KLK 4 antigens, the anti-KLK 4 antibodies specifically captured these antigens and caused changes in the electrochemical properties of the system. Randles equivalent circuit was utilized to evaluate the impedimetric signal, which was measured with the help of an electrochemical impedance spectroscopy method. After the specific interaction, the electron transfer resistance (R-ct) was remarkably increased and displayed a linear relationship with the level of the KLK 4 antigen in the range of 0.02-15 pg/mL, with a a detection limit of 6.8 fg/mL. The designed biosensor was able to detect a KLK 4 antigen with good sensitivity, excellent specificity, and high stability. In addition, because of having a low-cost and robust procedure for fabrication, it could be repeatedly used in several areas including clinical diagnosis.
dc.description.sponsorshipNamik Kemal University, NKU-BAP [NKUBAP.00.ONAP. 19.209]
dc.description.sponsorshipThe authors thank the Scientific and Technological Research Council of Tekirdag. Namik Kemal University, NKU-BAP (NKUBAP.00.ONAP. 19.209) for the support of this work.
dc.identifier.doi10.1021/acsbiomaterials.0c01602
dc.identifier.endpage1201
dc.identifier.issn2373-9878
dc.identifier.issue3
dc.identifier.pmid33583176
dc.identifier.scopus2-s2.0-85102658104
dc.identifier.scopusqualityN/A
dc.identifier.startpage1192
dc.identifier.urihttps://doi.org/10.1021/acsbiomaterials.0c01602
dc.identifier.urihttps://hdl.handle.net/20.500.13099/1920
dc.identifier.volume7
dc.identifier.wosWOS:000627588400032
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofAcs Biomaterials Science & Engineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250316
dc.subjecttetracarboxylic acid
dc.subjectkallikrein-related peptidase 4
dc.subjectmetallophthalocyanines
dc.subjectelectrochemical impedance spectroscopy
dc.subjectbiosensor
dc.titleDetection of Kallikrein-Related Peptidase 4 with a Label-free Electrochemical Impedance Biosensor Based on a Zinc(II) Phthalocyanine Tetracarboxylic Acid-Functionalized Disposable Indium Tin Oxide Electrode
dc.typeArticle

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