Horseradish Peroxidase Immobilized onto Mesoporous Magnetic Hybrid Nanoflowers for Enzymatic Decolorization of Textile Dyes: A Highly Robust Bioreactor and Boosted Enzyme Stability

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dc.authoridOZDEMIR, NALAN/0000-0002-8930-5198
dc.authoridAtes, Burhan/0000-0001-6080-229X
dc.contributor.authorBakar, Busra
dc.contributor.authorAkbulut, Mustafa
dc.contributor.authorUlusal, Fatma
dc.contributor.authorUlu, Ahmet
dc.contributor.authorOzdemir, Nalan
dc.contributor.authorAtes, Burhan
dc.date.accessioned2025-03-17T12:25:54Z
dc.date.available2025-03-17T12:25:54Z
dc.date.issued2024
dc.departmentTarsus Üniversitesi
dc.description.abstractRecently, hybrid nanoflowers (hNFs), which are accepted as popular carrier supports in the development of enzyme immobilization strategies, have attracted much attention. In this study, the horseradish peroxidase (HRP) was immobilized to mesoporous magnetic Fe3O4-NH2 by forming Schiff base compounds and the HRP@Fe3O4-NH2/hNFs were then synthesized. Under optimal conditions, 95.0% of the available HRP was immobilized on the Fe3O4-NH2/hNFs. Structural morphology and characterization of synthesized HRP@Fe3O4-NH2/hNFs were investigated. The results demonstrated that the average size of HRP@Fe3O4-NH2/hNFs was determined to be around 220 nm. The zeta-potential and magnetic saturation values of HRP@Fe3O4-NH2/hNFs were -33.58 mV and similar to 30 emu/g, respectively. Additionally, the optimum pH, optimum temperature, thermal stability, kinetic parameters, reusability, and storage stability were examined. It was observed that the optimum pH value shifted from 5.0 to pH 8.0 after immobilization, while the optimum temperature shifted from 30 to 80 degrees C. K-m values were calculated to be 15.5502 and 7.6707 mM for free HRP and the HRP@Fe3O4-NH2/hNFs, respectively, and V-max values were calculated to be 0.0701 and 0.0038 mM min(-1). The low K-m value observed after immobilization indicated that the affinity of HRP for its substrate increased. The HRP@Fe3O4-NH2/hNFs showed higher thermal stability than free HRP, and its residual activity after six usage cycles was approximately 45%. While free HRP lost all of its activity within 120 min at 65 degrees C, the HRP@Fe3O4-NH2/hNFs retained almost all of its activity during the 6 h incubation period at 80 degrees C. Most importantly, the HRP@Fe3O4-NH2/hNFs demonstrated good potential efficiency for the biodegradation of methyl orange, phenol red, and methylene blue dyes. The HRP@Fe3O4-NH2/hNFs were used for a total of 8 cycles to degrade methyl orange, phenol red, and methylene blue, and degradation of around 81, 96, and 56% was obtained in 8 h, respectively. Overall, we believe that the HRP@Fe3O4-NH2/hNFs reported in this work can be potentially used in various industrial and environmental applications, particularly for the biodegradation of recalcitrant compounds, such as textile dyes.
dc.description.sponsorshipErciyes ?niversitesi [100/2000]; Inonu University and Erciyes University; Council of Higher Education PhD Scholar Biomaterial and Tissue Engineering subdivision
dc.description.sponsorshipThe research work was supported by Inonu University and Erciyes University. Busra Bakar is a 100/2000 the Council of Higher Education PhD Scholar Biomaterial and Tissue Engineering subdivision.
dc.identifier.doi10.1021/acsomega.4c00703
dc.identifier.endpage24573
dc.identifier.issn2470-1343
dc.identifier.issue23
dc.identifier.pmid38882139
dc.identifier.scopus2-s2.0-85195097441
dc.identifier.scopusqualityQ1
dc.identifier.startpage24558
dc.identifier.urihttps://doi.org/10.1021/acsomega.4c00703
dc.identifier.urihttps://hdl.handle.net/20.500.13099/1917
dc.identifier.volume9
dc.identifier.wosWOS:001235242200001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.relation.ispartofAcs Omega
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250316
dc.subjectSilver Nanoparticles
dc.subjectDegradation
dc.subjectTitanium
dc.subjectChitosan
dc.titleHorseradish Peroxidase Immobilized onto Mesoporous Magnetic Hybrid Nanoflowers for Enzymatic Decolorization of Textile Dyes: A Highly Robust Bioreactor and Boosted Enzyme Stability
dc.typeArticle

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