Selective Photokilling of Human Pancreatic Cancer Cells Using Cetuximab-Targeted Mesoporous Silica Nanoparticles for Delivery of Zinc Phthalocyanine

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dc.authoridOcakoglu, Kasim/0000-0003-2807-0425
dc.authoridBresoli-Obach, Roger/0000-0002-7819-7750
dc.authoridEr, Ozge/0000-0003-3958-6726
dc.authoridNonell, Santi/0000-0002-8900-5291
dc.authoridInce, Mine/0000-0002-9164-0446
dc.authoridcolak, suleyman gokhan/0000-0002-4978-1499
dc.authoridYurt, Fatma/0000-0002-9394-6908
dc.contributor.authorEr, Ozge
dc.contributor.authorColak, Suleyman Gokhan
dc.contributor.authorOcakoglu, Kasim
dc.contributor.authorInce, Mine
dc.contributor.authorBresoli-Obach, Roger
dc.contributor.authorMora, Margarita
dc.contributor.authorLluisa Sagrista, Maria
dc.date.accessioned2025-03-17T12:25:14Z
dc.date.available2025-03-17T12:25:14Z
dc.date.issued2018
dc.departmentTarsus Üniversitesi
dc.description.abstractBackground: Photodynamic therapy (PDT) is a non-invasive and innovative cancer therapy based on the photodynamic effect. In this study, we sought to determine the singlet oxygen production, intracellular uptake, and in vitro photodynamic therapy potential of Cetixumab-targeted, zinc(II) 2,3,9,10,16,17,23,24-octa(tert-butylphenoxy))phthalocyaninato(2-)-N-29,N-30,N-31,N-32 (ZnPcOBP)-loaded mesoporous silica nanoparticles against pancreatic cancer cells. Results: The quantum yield (Phi(Delta)) value of ZnPcOBP was found to be 0.60 in toluene. In vitro cellular studies were performed to determine the dark- and phototoxicity of samples with various concentrations of ZnPcOBP by using pancreatic cells (AsPC-1, PANC-1 and MIA PaCa-2) and 20, 30, and 40 J/cm(2) light fluences. No dark toxicity was observed for any sample in any cell line. ZnPcOBP alone showed a modest photodynamic activity. However, when incorporated in silica nanoparticles, it showed a relatively high phototoxic effect, which was further enhanced by Cetuximab, a monoclonal antibody that targets the Epidermal Growth Factor Receptor (EGFR). The cell-line dependent photokilling observed correlates well with EGFR expression levels in these cells. Conclusions: Imidazole-capped Cetuximab-targeted mesoporous silica nanoparticles are excellent vehicles for the selective delivery of ZnPcOBP to pancreatic cancer cells expressing the EGFR receptor. The novel nanosystem appears to be a suitable agent for photodynamic therapy of pancreatic tumors.
dc.description.sponsorshipMinisterio de Economia y Competitividad [CTQ2016-78454-C2-1-R]; Scientific and Technological Research Council of Turkey (TUBITAK) [2214/A]; Ege University, Scientific Research Project (BAP) [16 NBE 001]; European Social Funds; SUR del DEC de la Generalitat de Catalunya [2017 FI_B2 00140]
dc.description.sponsorshipThis research was funded by Ministerio de Economia y Competitividad (grant number CTQ2016-78454-C2-1-R), the Scientific and Technological Research Council of Turkey (TUBITAK; grant number 2214/A), Ege University, Scientific Research Project (BAP; grant Number: 16 NBE 001), and the European Social Funds and the SUR del DEC de la Generalitat de Catalunya (grant number 2017 FI_B2 00140).
dc.identifier.doi10.3390/molecules23112749
dc.identifier.issn1420-3049
dc.identifier.issue11
dc.identifier.pmid30355983
dc.identifier.scopus2-s2.0-85055611735
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/molecules23112749
dc.identifier.urihttps://hdl.handle.net/20.500.13099/1572
dc.identifier.volume23
dc.identifier.wosWOS:000451641900021
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofMolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250316
dc.subjectZn(II) phthalocyanine
dc.subjectmesoporous silica nanoparticles
dc.subjectCetuximab
dc.subjectsinglet oxygen
dc.subjectphotodynamic therapy
dc.titleSelective Photokilling of Human Pancreatic Cancer Cells Using Cetuximab-Targeted Mesoporous Silica Nanoparticles for Delivery of Zinc Phthalocyanine
dc.typeArticle

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