A rational approach for 3D recognition and removal of L-asparagine via molecularly imprinted membranes

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dc.authoridOsman, Bilgen/0000-0001-8406-149X
dc.authoridDIKICI, EMRAH/0000-0002-3086-8156
dc.contributor.authorAcet, Omur
dc.contributor.authorNoma, Samir Abbas Ali
dc.contributor.authorAcet, Burcu Onal
dc.contributor.authorDikici, Emrah
dc.contributor.authorOsman, Bilgen
dc.contributor.authorOdabasi, Mehmet
dc.date.accessioned2025-03-17T12:27:03Z
dc.date.available2025-03-17T12:27:03Z
dc.date.issued2023
dc.departmentTarsus Üniversitesi
dc.description.abstractIn this study, a L-asparagine (L-Asn) imprinted membranes (L-Asn-MIPs) were synthesized via molecular imprinting for selective and efficient removal of L-Asn. The L-Asn-MIP membrane was prepared by using acrylamide (AAm) and hydroxyethyl methacrylate (HEMA) as a functional monomer and a comonomer, respectively. The membrane was characterized by scanning electron microscopy (SEM) and Fourier Transform infrared spectroscopy (FTIR). The L-Asn adsorption capacity of the membrane was investigated in detail. The maximum L-Asn adsorption capacity was determined as 408.2 mg/g at pH: 7.2, 24 degrees C. Determination of L-Asn binding behaviors of L-Asn-MIPs also shown with Scatchard analyses. The effect of pH on L-Asn adsorption onto the membrane and also the selectivity and reusability of the L-Asn-MIPs for L-Asn adsorption were determined through L-asparaginase (L-ASNase) enzyme activity measurements. The selectivity of the membrane was investigated by using two different ternary mixtures; L-glycine (L-Gly)/L-histidine (L-His)/L-Asn and L-tyrosin (L-Tyr)/L-cystein(L-Cys)/L-Asn. The obtained results showed that the L-Asn-MIP membranes have a high selectivity towards L-Asn.
dc.identifier.doi10.1016/j.jpba.2023.115250
dc.identifier.issn0731-7085
dc.identifier.issn1873-264X
dc.identifier.pmid36657352
dc.identifier.scopus2-s2.0-85146456162
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jpba.2023.115250
dc.identifier.urihttps://hdl.handle.net/20.500.13099/2041
dc.identifier.volume226
dc.identifier.wosWOS:000971451800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250316
dc.subjectAsparagine
dc.subjectL-asparaginase activity
dc.subjectMolecular imprinting
dc.subjectRemoval
dc.titleA rational approach for 3D recognition and removal of L-asparagine via molecularly imprinted membranes
dc.typeArticle

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