Cytotoxic effect of silica nanoparticles on human retinal pigment epithelial cells

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dc.authoridyilmaz, sakir necat/0000-0003-1759-3052
dc.authoridComelekoglu, Ulku/0000-0001-8060-6333
dc.authoridErat, Selma/0000-0001-7187-7668
dc.contributor.authorKaynar, Ayse Humeyra
dc.contributor.authorComelekoglu, Ulku
dc.contributor.authorKibar, Deniz
dc.contributor.authorYildirim, Metin
dc.contributor.authorYildirimcan, Saadet
dc.contributor.authorYilmaz, Sakir Necat
dc.contributor.authorErat, Selma
dc.date.accessioned2025-03-17T12:27:26Z
dc.date.available2025-03-17T12:27:26Z
dc.date.issued2023
dc.departmentTarsus Üniversitesi
dc.description.abstractIn recent years, the use of nanotechnology-based methods has become widespread in the treatment of ocular diseases. Silica nanoparticles (SiO2 NPs) are most common used NPs in medical field due to their physicochemical properties. SiO2 NPs can easily cross biological membranes and interact with basic biological structures, causing structural and functional changes in cells. In this study, it was aimed to investigate the dose dependent effect of SiO2 NPs on retinal pigment epithelium (RPE) in vitro using electrobiophysical, biochemical and histological methods. A commercially purchased human RPE (hARPE-19) cell line was used in this study. Cells were divided into four groups as control, 50 mg/mL SiO2, 100 mg/mL SiO2 and 150 mg/mL SiO2 groups. Cell index, apoptotic activity, cell cycle and oxidative stress markers were measured in all groups. Findings in the present study showed that SiO2 nanoparticles reduced cell proliferation, increased oxidative stress, apoptosis and arrest in the G0/G1 phase of the cell cycle as dose dependent manner in ARPE-19 cells. In conclusion, SiO2 exposure can induce cytotoxic effects in RPE cell line. The results of this study provide clues that exposure to SiO2 nanoparticles may impair visual function and reduce quality of life. However, further studies are needed in this regard.& COPY; 2023 Elsevier Inc. All rights reserved.
dc.description.sponsorshipScienti fic and Technological Research Council of Turkey [119S076]
dc.description.sponsorshipThis work was supported by The Scienti fic and Technological Research Council of Turkey (GrantNumber: 119S076) .
dc.identifier.doi10.1016/j.bbrc.2023.06.083
dc.identifier.endpage61
dc.identifier.issn0006-291X
dc.identifier.issn1090-2104
dc.identifier.pmid37406486
dc.identifier.scopus2-s2.0-85163813879
dc.identifier.scopusqualityQ2
dc.identifier.startpage53
dc.identifier.urihttps://doi.org/10.1016/j.bbrc.2023.06.083
dc.identifier.urihttps://hdl.handle.net/20.500.13099/2254
dc.identifier.volume674
dc.identifier.wosWOS:001029048400001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250316
dc.subjectARPE-19
dc.subjectCytotoxicity
dc.subjectOxidative stress
dc.subjectApoptosis
dc.subjectCell cycle
dc.subjectSilica nanoparticle
dc.titleCytotoxic effect of silica nanoparticles on human retinal pigment epithelial cells
dc.typeArticle

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