Imaging and detection of cell apoptosis by In vitro photodynamic therapy applications of zinc (II) phthalocyanine on human melanoma cancer

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dc.authoridYUZER, ABDULCELIL/0000-0002-2287-4126
dc.authoridYurt, Fatma/0000-0002-9394-6908
dc.contributor.authorZeinali, Sevda
dc.contributor.authorTuncel, Ayca
dc.contributor.authorYuzer, Abdulcelil
dc.contributor.authorYurt, Fatma
dc.date.accessioned2025-03-17T12:26:00Z
dc.date.available2025-03-17T12:26:00Z
dc.date.issued2021
dc.departmentTarsus Üniversitesi
dc.description.abstractThis study aims to investigate the photodynamic therapy (PDT) effects on MeWo (human melanoma cells) and HaCaT (normal human keratinocyte cells) by light stimulation of different concentrations of Zinc (II)-tetra-tertbutyl-phthalocyaninato (ZnPc). MTT viability assay data indicated that a 25 mu M concentration of ZnPc is cytotoxic to the melanoma cancer cells while this concentration of ZnPc is not cytotoxic for the HaCaT cell line. Moreover, the results showed that photoactivated ZnPc at 12.5 mu M concentration reduced the cell viability of the MeWo cell line to about 50 %. At this photosensitizing concentration, the efficacy of light doses of 20, 30, 40, and 50 J/cm(2) was evaluated against MeWo and HaCaT cells. ZnPc at a concentration of 12.5 mu M activated with a light dose of 50 J/cm(2) was the most efficient for the killing of MeWo cells. In conclusion, the 12.5 mu M of ZnPc with the treatment light dose of 50 J/cm(2) from a RED light source was adequate to destroy MeWo cells by the ROS-induced apoptosis mechanism. It also exhibited low killing effects on healthy HaCaT cells. These findings are supported by the results of apoptosis with the Annexin V & Dead Cell Kit and fluorescence imaging.
dc.identifier.doi10.1016/j.pdpdt.2021.102518
dc.identifier.issn1572-1000
dc.identifier.issn1873-1597
dc.identifier.pmid34478898
dc.identifier.scopus2-s2.0-85114610592
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.pdpdt.2021.102518
dc.identifier.urihttps://hdl.handle.net/20.500.13099/1989
dc.identifier.volume36
dc.identifier.wosWOS:000709143800002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250316
dc.subjectPhotodynamic therapy
dc.subjectMelanoma
dc.subjectZinc phthalocyanine
dc.subjectHuman melanoma cells
dc.subjectNormal human keratinocyte cells
dc.titleImaging and detection of cell apoptosis by In vitro photodynamic therapy applications of zinc (II) phthalocyanine on human melanoma cancer
dc.typeArticle

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