Development of mesoporous magnetic nanoparticles supported idarubicin and investigation of apoptotic and cytotoxic effects on cancer cell lines

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dc.authoridULUSAL, HASAN/0000-0003-3890-2088
dc.authoridOZDEMIR, NALAN/0000-0002-8930-5198
dc.authoridulusal, fatma/0000-0001-6926-6251
dc.contributor.authorUlusal, Hasan
dc.contributor.authorUlusal, Fatma
dc.contributor.authorOzdemir, Nalan
dc.date.accessioned2025-03-17T12:27:35Z
dc.date.available2025-03-17T12:27:35Z
dc.date.issued2024
dc.departmentTarsus Üniversitesi
dc.description.abstractBackgroundMany methods are used for cancer treatment, especially chemotherapy. In addition to the their therapeutic effects, chemotherapeutic drugs also have serious disadvantages, such as not being cell and tissue-specific, causing toxicity in many tissues, and developing drug resistance. Many methods, especially nanocarriers, have been designed to overcome these disadvantages.Methods and resultsIn this study, we synthesized mesoporous silica iron oxide nanoparticles with different pore diameters and loaded idarubicin (6MFe3O4-NH2-IDA and 35MFe3O4-NH2-IDA). The synthesized molecules were characterized using FT-IR, XRD, and SEM methods. The cytotoxic effects of unbound idarubicin and idarubicin-loaded nanoparticles on MCF7 and HL-60 cell lines were examined by MTT test. Additionally, the expression of anti-apoptotic (Survivin and BCL-2) and apoptotic (BAX, PUMA, and NOXA) genes of the nanoparticles were measured by PCR method. As a result of the analyses, it was seen that nanoparticles with the desired properties and sizes were synthesized. In MTT analysis, it was observed that both nanoparticles dramatically decreased the IC50 value in cell lines. However, the 35MFe3O4-NH2-IDA molecule was found to have lower IC50 values. IC50 values for pristine IDA, 6MFe3O4-NH2, and 35MFe3O4-NH2 at 24 h were found to be 3.56, 1.24 and 0.25 mu M in the MCF7 cell line and 4.15, 1.16 and 0.34 mu M in the HL-60 cell line, respectively. Additionally, apoptotic gene expression increased, and anti-apoptotic gene expression decreased.ConclusionsOur study demonstrates that the effectiveness of idarubicin can be significantly enhanced by its application with mesoporous nanocarriers. This enhancement is attributed to the controlled release of idarubicin from the nanocarrier, which circumvents drug resistance mechanisms, improves drug solubility, and increases the drug-carrying capacity per unit volume due to the porous structure of the carrier. These findings underscore the potential of the synthesized nanocarrier in cancer treatment and provide a clear direction for future research in this field.
dc.description.sponsorshipTrkiye Bilimsel ve Teknolojik Arascedil;timath;rma Kurumu
dc.description.sponsorshipThis manuscript was edited and proofread for correct English grammar, spelling, punctuation, and vocabulary at the Erciyes University Proofreading & Editing Office. We would like to thank the Proofreading & Editing Office of the Dean for Research at Erciyes University for copyediting and proofreading service for this manuscript.
dc.identifier.doi10.1007/s11033-024-09914-7
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue1
dc.identifier.pmid39259442
dc.identifier.scopus2-s2.0-85203549488
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s11033-024-09914-7
dc.identifier.urihttps://hdl.handle.net/20.500.13099/2332
dc.identifier.volume51
dc.identifier.wosWOS:001310608600003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250316
dc.subjectMesoporous magnetic nanoparticles
dc.subjectIdarubicin
dc.subjectCytotoxicity
dc.subjectCancer cell lines
dc.titleDevelopment of mesoporous magnetic nanoparticles supported idarubicin and investigation of apoptotic and cytotoxic effects on cancer cell lines
dc.typeArticle

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