Yazar "Ulusal, Hasan" seçeneğine göre listele

Listeleniyor 1 - 6 / 6
  • [ X ]
    Öğe
    A magnetically recyclable yolk-shell Au nanocatalyst for catalytic reduction of nitroaromatics and investigation of cytotoxicity on healthy and cancerous cell lines
    (Elsevier, 2025) Ulusal, Hasan; Ulusal, Fatma
    This study aimed to extract gold from waste water produced during secondary gold extraction and create a yolkshell catalyst with a magnetic nanoparticle core. Cytotoxicity tests were performed on healthy and cancerous cells to evaluate the potential of these gold nanoparticles, made from waste gold, in drug delivery. TEM and SEM results showed that the gold nanoparticles were uniformly dispersed in the silica shell of the catalyst without agglomeration. Cytotoxicity tests on healthy human bronchial epithelial cells (BEAS-2B) and cancer cells (SHSY5Y and A549) were conducted to assess the viability and potential use of these nanoparticles in medical drug delivery. Nanoparticles were used at concentrations ranging from 1 to 1000 mu g/mL, and the effects were studied at both 24 and 48 hour exposures. It was found that nanoparticles were nontoxic at low concentrations (<50 g/ mL) but their toxicity increased depending on time and concentration. It has been observed that the cytotoxic effect is less, especially in healthy cells. It was also found that nanoparticles from wastewater were less toxic. Moreover, cytotoxicity studies revealed a direct correlation between the increasing concentration of magnetic gold nanoparticles (MNP-3APP-Au from pure gold, MNP-3APW-Au from waste gold) and their cytotoxic effects. In addition, catalytic reduction of the environmentally toxic 4-nitrophenol, one of the application areas of gold, in the presence of sodium boron hydride was also carried out in order to evaluate the other possible applications of waste gold. It can be said that the catalytic activities of MNP-3APP-Au nanoparticles (98.09 % at 90 min.) obtained from pure gold salt are slightly better than MNP-3APW-Au nanoparticles (97.5 % at 35 min). However, although it takes longer to complete the para nitrophenol conversion, it can be said that the activity of MNP3APW-Au nanoparticles is quite high compared to the literature data.
  • [ X ]
    Öğe
    Attachment of Idarubicin to Glutaraldehyde-coated Magnetic Nanoparticle and Investigation of its Effect in HL-60 Cell Line
    (2022) Ulusal, Hasan; Ulusal, Fatma; Bozdayi, Mehmet Akif; Güzel, Bilgehan; Taysı, Seyithan; Tarakçıoğlu, Mehmet
    Idarubicin is a chemotherapeutic drug frequently used to treat breast cancer and acute leukemia. This study aimed to immobilize idarubicin on glutaraldehyde (GA)-coated magnetic nanoparticles (MNP-GA) to prepare a drug with high stability and low toxicity. We prefreed MNPS because of their easy synthesis, low cost, and non-toxicity. In the study, magnetite (Fe3O4) nanoparticles were prepared, coated with glutaraldehyde, characterization processes were performed with Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction pattern (XRD), and Conventional transmission electron microscopy (C-TEM) methods, and idarubicin (IDA) was bound. The cytotoxic effects of idarubicin-bound MNP-GA and free idarubicin on HL-60 cell lines were determined by MTT and ATP tests, and IC50 values were calculated. Flow cytometry was used to evaluate apoptosis status, and the expression of MDR1, Puma, NOXA, BAX, Survivin, and BCL-2 genes were measured by the polymerase chain reaction (PCR). It was found that the IC50 decreased between 5 and 7 times with the use of MNP. In PCR tests, the expressions of apoptotic genes increased, while the expressions of MDR1 and anti-apoptotic genes were decreased in the use of MNP. Apoptosis was found to be increased in flow cytometry measurements. The use of MNP systems has reduced drug resistance since it provides controlled release of the drug and prevents its exit from the cell due to its structure.
  • [ X ]
    Öğe
    Development of mesoporous magnetic nanoparticles supported idarubicin and investigation of apoptotic and cytotoxic effects on cancer cell lines
    (Springer, 2024) Ulusal, Hasan; Ulusal, Fatma; Ozdemir, Nalan
    BackgroundMany methods are used for cancer treatment, especially chemotherapy. In addition to the their therapeutic effects, chemotherapeutic drugs also have serious disadvantages, such as not being cell and tissue-specific, causing toxicity in many tissues, and developing drug resistance. Many methods, especially nanocarriers, have been designed to overcome these disadvantages.Methods and resultsIn this study, we synthesized mesoporous silica iron oxide nanoparticles with different pore diameters and loaded idarubicin (6MFe3O4-NH2-IDA and 35MFe3O4-NH2-IDA). The synthesized molecules were characterized using FT-IR, XRD, and SEM methods. The cytotoxic effects of unbound idarubicin and idarubicin-loaded nanoparticles on MCF7 and HL-60 cell lines were examined by MTT test. Additionally, the expression of anti-apoptotic (Survivin and BCL-2) and apoptotic (BAX, PUMA, and NOXA) genes of the nanoparticles were measured by PCR method. As a result of the analyses, it was seen that nanoparticles with the desired properties and sizes were synthesized. In MTT analysis, it was observed that both nanoparticles dramatically decreased the IC50 value in cell lines. However, the 35MFe3O4-NH2-IDA molecule was found to have lower IC50 values. IC50 values for pristine IDA, 6MFe3O4-NH2, and 35MFe3O4-NH2 at 24 h were found to be 3.56, 1.24 and 0.25 mu M in the MCF7 cell line and 4.15, 1.16 and 0.34 mu M in the HL-60 cell line, respectively. Additionally, apoptotic gene expression increased, and anti-apoptotic gene expression decreased.ConclusionsOur study demonstrates that the effectiveness of idarubicin can be significantly enhanced by its application with mesoporous nanocarriers. This enhancement is attributed to the controlled release of idarubicin from the nanocarrier, which circumvents drug resistance mechanisms, improves drug solubility, and increases the drug-carrying capacity per unit volume due to the porous structure of the carrier. These findings underscore the potential of the synthesized nanocarrier in cancer treatment and provide a clear direction for future research in this field.
  • [ X ]
    Öğe
    Extraction of malva sylvestris seed oil by soxhlet method and alginate/ chitosan microencapsulation with a new method: Investigation of its cytotoxic effects on human neuroblastoma cell line (SH-SY5Y)
    (Elsevier, 2024) Ulusal, Hasan; Ulusal, Fatma; Dagli, Sibel; Toprak, Cahit
    Biopolymers such as chitosan or alginate are at the forefront in healthcare applications due to their broad bioactivities. Biomolecules obtained by loading plant extracts into capsules prepared with chitosan and alginate combinations show anticancer, antioxidant and antimicrobial properties. In this study, Malva sylvestris seed oil (MSSO) was extracted by Soxhlet extraction method and alginate/chitosan encapsulation was performed with a new method. The anticancer properties of the prepared capsules were examined in the human neuroblastoma cell line (SH-SY5Y). For this purpose, MSSO was separated by the maceration extraction (Extract 1) and soxhelet extraction (Extract 2) and the extracts were microencapsulated with alginate and chitosan (MicroCapsule 1 and MicroCapsule 2). Encapsulation efficiency (EE%) and drug loading (DL%) were calculated for each two capsules. The cytotoxic effects of the prepared microcapsules and extracts were examined with the CCK-8 cytotoxicity test on the SH-SY5Y cell line for 24 and 48 h of incubation periods. EE and DL for microcapsule 1 were 94.10% and 4.92%, respectively, and EE and DL for microcapsule 2 were 86.69% and 3.15%, respectively. IC50 values calculated using Graphad Prism 10.0.2. As a result of the encapsulation of the extracts, it was found that both capsules had approximately 5 times lower IC50 values. Additionally, lower IC50 values were found at 48 h of incubation due to the slow release of the extracts from the capsules. Since the microencapsulation process increases the amount of substance in the unit volume and provides a slow and controlled release of the drug, it provides cytotoxic effects at lower doses. This will allow the drug to be more concentrated in cancerous cells and tissues and overcome drug resistance that may develop in the cell.
  • [ X ]
    Öğe
    Imparting of Nearly Superparamagnetic Properties to Cryogel Scaffolds With Mesoporous MNPs for Magneto-Sensitive Tissue Engineering Strategies
    (Wiley, 2024) Demir, Didem; Ulusal, Fatma; Ulusal, Hasan; Ceylan, Seda; Dagli, Sibel; Ozdemir, Nalan; Tarakcioglu, Mehmet
    This work reports the assembly of mesoporous iron oxide nanoparticles (meso-MNPs) with cryogel scaffolds composed of chitosan and gelatin. Meso-MNPs with a particle size ranging from 2 and 50 nm, a surface area of 140.52 m2 g-1, and a pore volume of 0.27 cm3 g-1 were synthesized on a porous SiO2 template in the presence of PEG 6000 followed by leaching of SiO2. Different ratios of meso-MNPs were successfully incorporated into chitosan:gelatin cryogels up to an amount equivalent to the entire amount of polymer. The morphological structure and physicochemical properties of the cryogels were directly affected by the amount of MNPs. VSM curves showed that all composite cryogels could be magnetized by applying a magnetic field. In the context of the safety of magnetic cryogel scaffolds for use in biomedicine, it is important to note that all values are below the exposure limit for static magnetic fields, and according to cytotoxicity data, scaffolds containing meso-MNPs showed nontoxicity with cell viability ranging from 150% to 275%. In addition, microbial analysis with gram-negative and gram-positive bacteria showed that the scaffolds exhibited activity against these bacteria. image
  • [ X ]
    Öğe
    Investigation of in vitro toxicity of newly synthesized mesoporous silica nanotubes with different pore sizes in human liver cancer cells (HepG2)
    (İbrahim DEMİRTAŞ, 2024) Ulusal, Hasan; Ulusal, Fatma
    Nanotechnology has gained importance in recent years with the use of nanomaterials smaller than human cells in many areas such as food, cosmetics, defense industry and pharmaceutical industry. It has begun to be widely used in the field of health in the diagnosis and treatment of many diseases, especially cancer. However, due to their size and content, these materials can be toxic and pose a risk to human health. In this study, the cytotoxic effects of mesoporous silicon dioxide (SiO2) nanoparticles with different pore sizes, synthesized using a new method and made from polyethylene glycol 6000 (PEG6000) and polyethylene glycol 35000 (PEG35000) were tested on HepG2 cells liver carcinoma cells. Additionally, the effects of mesoporous silica nanotubes on lipid peroxidation and reactive oxygen species (ROS) were also examined. It was found that the cytotoxicity of both types of mesoporous SiO2 nanoparticles increased with rising concentration. Cell viability decreased significantly as the nanoparticles dosage (100-10 µg/mL) increased. Both nanoparticles were not cytotoxic at concentrations up to 50 µg/mL, however, they became cytotoxic at higher concentrations (p