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Öğe Antifouling and antibacterial performance evaluation of polyethersulfone membranes modified with AZ63 alloy(Iwa Publishing, 2023) Ozay, Yasin; Alterkaoui, Aya; Kahya, Kursat; Ozdemir, Sadin; Gonca, Serpil; Dizge, Nadir; Ocakoglu, KasimAntibacterial membranes have attracted researchers' interest in recent years as a possible approach for dealing with biofouling on the membrane surface. This research aims to see if blending AZ63 Mg alloy into a polyethersulphone (PES) membrane can improve antifouling and separation. The composite membranes' pure water flux continued to increase from pristine PES to PES/AZ63 2.00 wt%. The results showed that PES/AZ63 2.00 wt% membrane supplied the highest permeate flux of E. coli. The steady-state fluxes of AZ63 composite membranes were 113.24, 104.38 and 44.79 L/m(2)h for PES/AZ63 2.00 wt%, 1.00 wt%, and 0.50 wt%, respectively. The enhanced biological activity of AZ63 was studied based on antioxidant activity, DNA cleavage, antimicrobial, anti-biofilm, bacterial viability inhibition and photodynamic antimicrobial therapy studies. The maximum DPPH scavenging activity was determined as 81.25% with AZ63. AZ63 indicated good chemical nuclease activity and also showed moderate antimicrobial activity against studied strains. The highest biofilm inhibition of AZ63 was 83.25% and 71.63% towards P. aeruginosa and S. aureus, respectively. The cell viability inhibition activity of AZ63 was found as 96.34% against E. coli. The photodynamic antimicrobial therapy results displayed that AZ63 demonstrated 100% bacterial inhibition when using E. coli.Öğe Antimicrobial and antioxidant activity of phenolic extracts from walnut (Juglans regia L.) green husk by using pressure-driven membrane process(Springer India, 2023) Arslan, Hudaverdi; Koc, Eda Ondul; Ozay, Yasin; Canli, Oltan; Ozdemir, Sadin; Tollu, Gulsah; Dizge, NadirIn this study, antioxidant (DPPH and metal chelating), DNA cleavage, biofilm, and antimicrobial properties of extracted phenol from the walnut green husk (WGH) and its different concentrate and permeate samples were evaluated. For maximum phenolic compound extraction from the WGH first, the effects of solvent type (deionized water, methanol, n-hexane, acetone, and ethanol), solvent temperature (25-75 degrees C), and extraction time (0.5-24 h) were optimized. Then to concentrate phenolic compounds a pressure-driven membrane process was used with four different membrane types. The phenol contents of the concentrate samples were found to be microfiltration (MF) concentrate 4400 mg/L, ultrafiltration (UF) concentrate 4175 mg/L, nanofiltration (NF) concentrate 8155 mg/L, and reverse osmosis (RO) concentrate 8100 mg/L. LC-MSMS was used to determine the quantification of phenolic compounds in permeate and concentrate streams. In addition, all of the concentrate samples with high phenol content showed a high antioxidant activity as 100% with MF concentrate, UF concentrate, NF concentrated and RO concentrated. Likewise, concentrate samples were found to have very high antibiofilm activity as 82.86% for NF concentrate againts S. aureus, 85.80% for NF concentrate against P. aureginosa, 80.95% for RO concentrate against S. aureus, and 83.61% for RO-concentrate against P. aureginosa. When the antimicrobial activity of the extracted phenol from WGH and its different concentrate and permeate samples were evaluated by micro dilution and disk diffusion methods, it was found that the ability of the concentrate samples to inhibit bacterial growth was much higher than permeate ones. In addition, extracted phenol from WGH and its different concentrate and permeate samples showed significant DNA nuclease activity.Öğe Antimicrobial Effects of Nanostructured Rare-Earth-Based Orthovanadates(Springer, 2022) Gonca, Serpil; Yefimova, Svetlana; Dizge, Nadir; Tkachenko, Anton; Ozdemir, Sadin; Prokopiuk, Volodymyr; Klochkov, VladimirThe search for novel antimicrobial agents is of huge importance. Nanomaterials can come to the rescue in this case. The aim of this study was to assess the cytotoxicity and antimicrobial effects of rare-earth-based orthovanadate nanoparticles. The cytotoxicity against host cells and antimicrobial activity of LaVO4:Eu3+ and GdVO4:Eu3+ nanoparticles were analyzed. Effects of nanomaterials on fibroblasts were assessed by MTT, neutral red uptake and scratch assays. The antimicrobial effects were evaluated by the micro-dilution method estimating the minimum inhibitory concentration (MIC) of nanoparticles against various strains of microorganisms, DNA cleavage and biofilm inhibition. GdVO4:Eu3+ nanoparticles were found to be less toxic against eukaryotic cells compared with LaVO4:Eu3+. Both nanoparticles exhibited antimicrobial activity and the highest MIC values were 64 mg/L for E. hirae, E. faecalis and S. aureus shown by GdVO4:Eu3+ nanoparticles. Nanoparticles demonstrated good DNA cleavage activity and induction of double-strand breaks in supercoiled plasmid DNA even at the lowest concentrations used. Both nanoparticles showed the biofilm inhibition activity against S. aureus at 500 mg/L and reduced the microbial cell viability. Taken the results of host toxicity and antimicrobial activity studies, it can be assumed that GdVO4:Eu3+ nanoparticles are more promising antibacterial agents compared with LaVO4:Eu3+ nanoparticles.Öğe Electrospun Composite Nanofibers Based on Poly (epsilon-Caprolactone) and Styrax Liquidus (Liquidambar orientalis Miller) as a Wound Dressing: Preparation, Characterization, Biological and Cytocompatibility Results(Springer, 2022) Demir, Didem; Ozdemir, Sadin; Ceylan, Seda; Yalcin, M. Serkan; Sakim, Burcu; Bolgen, NimetIn this study, styrax liquidus (sweet gum balsam) extracted from Liquidambar orientalis Mill. incorporated PCL fibrous scaffolds were prepared using the electrospinning method. The effects of the styrax liquidus content on the prepared scaffolds were investigated using different physico-chemical and morphological analyses. Then, the styrax-loaded nanofibers were examined for their antioxidant activity, anti-biofilm, metal chelating, antimicrobial and DNA cleavage properties. The results obtained from these studies showed that the nanofibers exhibited effective biological activity depending on the weight ratio of the styrax liquidus. In light of the data obtained from the characterization and biological studies, a sample with high ratio of balsam was built for determining the cytocompatibility analysis in vitro. The cytotoxicity studies of the selected membrane were conducted using mouse embryonic fibroblast cells. The fibrous scaffolds lead to increase the cell number as a result of high viability. According to the results, we propose a novel biocompatible electrospun hybrid scaffold with antioxidant and antimicrobial properties that can be used as wound healing material for potential tissue engineering applications.Öğe Experimental confirmation of antimicrobial effects of GdYVO4:Eu3+ nanoparticles(Taylor & Francis Ltd, 2021) Gonca, Serpil; Ozdemir, Sadin; Yefimova, Svetlana; Tkachenko, Anton; Onishchenko, Anatolii; Klochkov, Vladimir; Kavok, NataliyaNanotechnology can be applied to design antibacterial agents to combat antibiotic resistance. The aim of the present study was to assess the antimicrobial effects and cytotoxicity of GdYVO4:Eu3+ nanoparticles (NPs). Biofilm inhibition activity, antimicrobial activity, bacterial viability inhibition and DNA cleavage activity of GdYVO4:Eu3+ NPs were studied. In addition, the impact of GdYVO4:Eu3+ NPs on the mitochondrial membrane potential (Delta psi(M)) of host immune cells and, hence, their apoptosis was analyzed by JC-1 staining using flow cytometry. GdYVO4:Eu3+ NPs demonstrated good antimicrobial, cell viability inhibition and DNA cleavage activities. In addition, GdYVO4:Eu3+ NPs showed good biofilm inhibition activity against S. aureus and P. aeruginosa and inhibition percentages were 89.15% and 79.54%, respectively. However, GdYVO4:Eu3+ NPs promoted mitochondrial depolarization and apoptosis of leukocytes at high concentrations. GdYVO4:Eu3+ nanoparticles are promising antibacterial agents. However, more efforts should be exerted to ensure their safety.Öğe Fabrication of Ag nanoparticles coated leonardite basalt ceramic membrane with improved antimicrobial properties for DNA cleavage, E. coli removal and antibiofilm effects(Elsevier Science Inc, 2023) Saleh, Mohammed; Isik, Zelal; Belibagli, Pinar; Arslan, Hudaverdi; Gonca, Serpil; Ozdemir, Sadin; Kudaibergenov, NurbolatThis study aimed to fabricate a novel, low-cost, and environmental-friendly ceramic membrane based on basalt and leonardite powders via press and sintering methods. The fabricated leonardite basalt ceramic membrane (LBCM) was coated with silver nanoparticles (AgNPs); to create an antibacterial surface. The capabilities of the bare and coated membranes were examined. In this context, water permeability reached 554 +/- 2.61 and 447 +/- 1.21 L/m2hbar for bare LBCM and AgNPs-coated LBCM, respectively. The fabricated membranes indicated 100% Escherichia coli (E. coli) removal efficiency at a transmembrane pressure of 0.5 bar. The solid surface antimicrobial activity of the LBCM and AgNPs-coated LBCM reached 26.38% and 100%, respectively. The LBCM and AgNPs-coated LBCM were analyzed for the in-vitro 2,2diphenyl-1-picrylhydrazyl (DPPH) antioxidant. Accordingly, AgNPs-coated LBCM exhibited higher antioxidant activities than raw LBCM. The scavenging capacity reached 83.91% with AgNPs-coated LBCM, while only 58.95% was achieved with raw LBCM, indicating that AgNPs-coated LBCM was better than bare LBCM from an antioxidant activities perspective. AgNPs-coated LBCM had a deoxyribonucleic acid (DNA) cleavage activity (single-strand DNA cleavage activity at 50 mg/L and double-strand DNA cleavage activity at 100 and 200 mg/L). In contrast, the raw LBCM did not exhibit DNA cleavage activity at any concentration. AgNPs-coated LBCM showed higher antimicrobial activities (minimum inhibition concentrations (MICs) were 32 mg/L against Enterococcus faecalis (E. faecalis) and 64 mg/L against Staphylococcus aureus (S. aureus), Candida tropicalis (C. tropicalis), and Enterococcus hirae (E. hirae)). The biofilm inhibition of LBCM and AgNPs-coated LBCM powders was tested against S. aureus and Pseudomonas aeruginosa (P. aeruginosa). The maximum S. aureus inhabitations by LBCM and AgNPcoated LBCM were 60.34% and 99.12%, respectively. The inhabitation of P. aeruginosa increased from 52.38% before coating to 96.37% at the end of the coating process. Regarding E.coli microbial cell viability inhibition, LBCM powders and AgNPs-coated LBCM powders were found to inhibit E. coli growth by 68.35% and 100%, respectively. (c) 2023 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.Öğe Improvement in performance of g-C3N4 nanosheets blended PES ultrafiltration membranes including biological properties(Elsevier, 2021) Sert, Buse; Ozay, Yasin; Harputlu, Ersan; Ozdemir, Sadin; Yalcin, M. Serkan; Ocakoglu, Kasim; Dizge, NadirThis study aims to investigate the modification of polyethersulphone (PES) membrane with graphitic carbon nitride (g-C3N4) nanosheets for improving the antifouling and separation performance. The nanocomposite membranes were fabricated with blending of different g-C3N4 nanosheets (0.50, 1.00, and 2.00 wt%) into PES and they were synthesized by the phase inversion method. The fabricated g-C3N4 nanosheets and composite membranes were analyzed for their morphology. Scanning electron microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDX) mapping were used to detect the distribution of g-C3N4 nanosheets on membrane surface, whereas surface roughness of membrane was evaluated by atomic force microscopy (AFM). The composite membrane surface was found to be hydrophilic (67.54 degrees), while the water flux of the composite membrane was found to be 254.8 L/m(2)/h for 2.00 wt% g-C3N4/PES membrane. The bovine serum albumin (BSA) separation tests indicated that the composite membrane supplied 98.5% BSA rejection ratio. Moreover, a significant improvement in antifouling characteristics were verified from BSA filtration experiments. g-C3N4 was also investigated for some of its biological properties such as antioxidant, antimicrobial, DNA cleavage, biofilm inhibition, and bacterial viability effect. g-C3N4 showed good free radical scavenging activity and moderate chelating activity at 500 mg/L. It was also determined that single-strand DNA cleavage activities occurred at all tested concentrations. g-C3N4 exhibited significant antibiofilm activity and inhibitory effects on E. coli vitality as 90.9%, 97.1%, and 98.9% at 250, 500, and 1000 mg/L, respectively. This study provides a simple and useful guideline to create a UF membrane resistant against organic fouling and expand its practical applications for wastewater treatment.Öğe Investigation of Antimicrobial Activity of Iron Oxide Nanoparticles (FeONPs) and Zinc Oxide Nanoparticles (ZnONPs) Produced with Aerial Part Extract of Cucumber(Springer, 2024) Alterkaoui, Aya; Eskikaya, Ozan; Ozdemir, Sadin; Yalcin, M. Serkan; Dizge, NadirOne of the most threatening factors to human and living health today, as in the past, is pathogens, which cause significant damage to health. Many materials are used to destroy such bacteria. The investigation of antibacterial properties of materials containing heavy metals produced with plant extracts continues. In this study, iron and zinc oxide nanoparticles (FeONPs and ZnONPs) were produced with cucumber aerial part extract. The nanoparticles have diameters ranging from 60 to 120 nm for FeONPs and 70 to 130 nm for ZnONPs. The antibacterial properties of the produced materials were investigated. The biological evaluation of the ZnONPs and FeONPs was also reported in this study. The antioxidant activity of ZnONPs and FeONPs was found 48.00%, and 40.65%, respectively, at 100 mg/L concentration. ZnONPs exhibited significantly antimicrobial activity than FeONPs. Both NPs showed excellent DNA nuclease activity and also E. coli cell viability was strongly inhibited at all tested doses. At 250 mg/L, ZnONPs inhibited P. aeruginosa and S. aureus biofilms by 90.1% and 94.0%, respectively. FeONPs also reduced biofilm formation against P. aeruginosa and S. aureus by 87.8% and 93.4% at 250 mg/L, respectively. However, when all the results were compared, it was observed that ZnONP had a slightly higher antioxidant effect than FeONPs. The antibacterial effect of cucumber, particularly extracted from its waste aerial parts, demonstrated promising potential in combating microbial infections. This effect is evidenced by the utilization of cucumber extract in the synthesis of nanoparticles, namely FeONPs and ZnONPs, which exhibited significant antibacterial properties. The study underscores the importance of exploring natural sources like cucumber in developing novel antimicrobial agents. Further research in this area could unveil additional mechanisms underlying cucumber's antibacterial activity and facilitate the development of effective antibacterial treatments derived from natural sources.Öğe Investigation of the antifouling properties of polyethersulfone ultrafiltration membranes by blending of boron nitride quantum dots(Elsevier, 2021) Sert, Buse; Gonca, Serpil; Ozay, Yasin; Harputlu, Ersan; Ozdemir, Sadin; Ocakoglu, Kasim; Dizge, NadirThis study aims to investigate the modification of polyethersulfone (PES) membrane with boron nitride quantum dots (BNQD) for improving the antifouling performance. The composite membranes were synthesized by blending different amounts of BNQD (0.50, 1.00, and 2.00 wt.%) into PES with the non-solvent induced phase separation (NIPS) method. UV-vis absorption, X-ray diffraction (XRD), and transmission electron microscopy (TEM) were used to characterize BNQD. Moreover, porosity, pore size, contact angle, permeability, bovine serum albumin (BSA) rejection, and antifouling properties were determined for composite membranes. The enhanced biological activity of BNQD was investigated based on antioxidant, antimicrobial, anti-biofilm, bacterial viability inhibition, and DNA cleavage studies. The BNQD showed 19.35 % DPPH radical scavenging activity and 76.45 % ferrous ion chelating activity at 500 mg/L. They also exhibited good chemical nuclease activity at all concentrations. BNQD had moderate antibacterial activity against all tested microorganisms. Biofilm inhibition percentage of BNQD was determined as 82.31 % at 500 mg/L. Cell viability assay demonstrated that the BNQD showed strong cell viability inhibition 99.9 % at the concentration of 1000 mg/L. The porosity increased from 56.83 +/- 1.17%-61.83 +/- 1.17 % while BNQD concentration increased from 0 to 2.00 wt%. Moreover, the hydrophilicity of BNQD nanocomposite membranes also increased from 75.42 +/- 0.56 degrees to 65.34 +/- 0.25 degrees. The mean pore radius is far slightly changed from 16.47 +/- 0.35 nm to 19.16 +/- 0.22 nm. The water flux increased from 133.5 +/- 9.5 L/m(2)/h (for pristine membrane) to 388.6 +/- 18.8 L/m(2)/h (for PES/BNQD 2.00 wt% membrane). BSA flux increased from 38.8 +/- 0.9 L/m(2)/h to 63.2 +/- 2.7 L/m(2)/h up to 1.00 wt% amount of BNQD nanoparticles.Öğe Investigation of the Effect of Sintering of Waste Welding Powder on Antioxidant and Antimicrobial Activity(Springer Int Publ Ag, 2022) Eskikaya, Ozan; Arslan, Hudaverdi; Ozdemir, Sadin; Gonca, Serpil; Dizge, NadirThe use of waste as a material that can be useful in other areas can reduce both waste generation and the need for resources. Welding powder with high heavy metal content should be used for other purposes. For this reason, the welding powder, which is frequently used in the metal industry and released after the welding process, is desired to be used as an agent against microorganisms in this study. Several biological activities of welding powder and its sintered forms also investigated. The welding powder and its sintered forms showed moderate antioxidant and antimicrobial activity. Welding powder without sintering is called WP. Welding powders sintered at 300 ?degrees C, 600?degrees C, and 900?degrees C are named as WP300, WP600, and WP900, respectively. DNA cleavage activity was tested, and it showed single-stand cleavage against pBR 322 plasmid DNA. They displayed excellent biofilm inhibition activity, and the biofilm inhibition of WP, WP300, WP600, and WP900 against S. aureus and P. aeruginosa were found as 99.34%, 99.21%, 98.99%, and 99.12% and 98.32%, 96.11%, 96.38%, and 95.39%, respectively, at concentrations of 500 mg/L, respectively. They also demonstrated significant cell viability activity against E. coli.Öğe Investigation of the treatment and antibacterial properties of pollutant-containing water using black phosphorus blended polyethersulfone membranes(Elsevier Ltd, 2024) Eskikaya, Ozan; Kucukosman, Rıdvan; Ozdemir, Sadin; Yalcin, M. Serkan; Ocakoglu, Kasım; Dizge, NadirThe most important problem of membrane processes is clogging and therefore the reduction of their useful life. In order to eliminate such problems, the production of composite membranes is an option in membrane treatment systems. In this study, the usability of black phosphorus (BP) in membrane treatment systems, and its effect on antibacterial and treatment performance were studied. BP blended PES membranes (BP@PES) produced by loading BP into the polyethersulfone (PES) membranes were used for the treatment of bovine serum albumin (BSA) and Escherichia coli (E. coli). For this purpose, BP was added at three different amounts (0.5 %, 1.0 %, and 2.0 %) into PES membranes. Membranes with different BP amounts produced were named as 0.5%BP@PES, 1.0%BP@PES, and 2.0%BP@PES. After the production of these composite membranes, they were used in the dead-end filtration system and compared with the PES membrane without BP (bare PES). The removal effectiveness of bare PES was found to be 53.40 % in the BSA rejection results, and it was discovered to be 60.90 %, 97.15 %, and 100 % for 0.5%BP@PES, 1.0%BP@PES, and 2.0%BP@PES, respectively. The antioxidant activity of BP was 70.86% at 100 mg/L. Newly prepared BP caused single-strand DNA nuclease abilities at 50, 100, and 200 mg/L. BP showed the effective antimicrobial activity. The most antimicrobial activity was determined as a minimum inhibitory concentration (MIC) value of 8 mg/L against E. hirae and 100 % E. coli viability inhibition activity was achieved at 50 and 100 mg/L after 90 min exposure. BP also inhibited the biofilm formation of P. aeruginosa at 87.19 % and S. aureus at 90.28 % at 50 mg/L. Thus, both the effect of BP on membrane performance and its antibacterial properties were investigated. Pure water fluxes, BSA and E.coli rejection performances, and antibacterial properties of all membranes used in the membrane filtration system were investigated in detail. It is recommended to further investigate BP-containing membranes with different production techniques.Öğe Investigation of two different size microplastic degradation ability of thermophilic bacteria using polyethylene polymers(Taylor & Francis Ltd, 2023) Akarsu, Ceyhun; Ozdemir, Sadin; Ozay, Yasin; Acer, Omer; Dizge, NadirThere are several studies stating that many types of microplastics cannot be retained completely by conventional wastewater treatment systems. Therefore, it is necessary to prevent the discharge of these microplastics to the ecological system. The objective of this study was to investigate the biodegradation ability of two different size of PE (50 and 150 mu m) by using two Gram-positive, spore-forming, rod-shaped, and motile thermophilic bacteria, called strain Gecek4 and strain ST5, which can hydrolyse starch, were isolated from the soil's samples of Gecek and omer hot-springs in Afyonkarahisar, Turkey, respectively. Phenotypic features and 16S rRNA analyzing of strains also studied. According to these results, Gecek4s and ST5 were identified as Anoxybacillus flavithermus Gecek4s and Bacillus firmus ST5, respectively. Results showed that A. flavithermus Gecek4s could colonise the polymer surface and cause surface damage whereas B. firmus ST5 could not degrade bigger-sized particles efficiently. In addition, morphological changes on microplastic surface were investigated by scanning electron microscopy (SEM) where dimensional changes, irregularities, crack, and/or holes were detected. This finding suggests that there is a high potential to develop an effective integrated method for plastic bags degradation by extracellular enzymes from bacteria.Öğe Nanocellulose synthesis from Tamarix aphylla and preparation of hybrid nanocellulose composites membranes with investigation of antioxidant and antibacterial effects(Elsevier, 2022) M'barek, Islem; Isik, Zelal; Ozay, Yasin; Ozdemir, Sadin; Tollu, Gulsah; Moussaoui, Younes; Dizge, NadirNanotechnology takes place in almost every stage of our lives and it has also started to find application in membrane technologies. In order to overcome the fouling phenomenon, which is the main problem of membrane processes, composite membranes containing metallic nanoparticles (NPs) with antibacterial properties are produced. Therefore, research continues to find environmentally friendly, inexpensive, and abundant materials to prevent membrane fouling. This study focused on the valorization of vegetable biomass Tamarix aphylla for nanocellulose (NC) synthesis which was coated subsequently with silver (NC-Ag NPs) and iron (NC-Fe NPs) nanoparticles to acquire the hybrid composites for ultrafiltration membrane preparation. The coated NC with metal NPs and synthesized membranes were characterized by SEM, EDX, FTIR, and XRD. The antioxidant and antimicrobial activities of NC and its coated forms with NPs were evaluated. It was found that significant scavenging abilities against the DPPH radical reached the maximum of 82.94% at 200 mg/L. The abilities of the three samples (NC, NC-Ag NPs, NC-Fe NPs) for DNA cleavage were tested and important outcomes were recorded. Furthermore, the antimicrobial abilities of the composites were examined by the microdilution procedure and they exhibited good ability. The biofilm inhibition rates of the composites were determined, where the highest biofilm inhibition against S. aureus and P. aeruginosa was recorded for NC-Ag NPs as 63.85% and 81.20%, respectively. Briefly, among the tested NC and NPs coated NC, NC-Fe NPs exhibited significant antimicrobial potential compared to the others. Thus, it may be promising hybrid nanocellulose complexes for antifouling membrane preparation.Öğe Polyethersulfone membranes modified with CZTS nanoparticles for protein and dye separation: Improvement of antifouling and self-cleaning performance(Elsevier, 2021) Ocakoglu, Kasim; Dizge, Nadir; Colak, Suleyman Gokhan; Ozay, Yasin; Bilici, Zeynep; Yalcin, M. Serkan; Ozdemir, SadinIn this study, a novel polyethersulphone (PES) nanocomposite membrane incorporated with copper zinc tin sulfide (Cu2ZnSnS4) nanoparticles (CZTS NPs) was prepared through the phase inversion method. First, the samples containing different copper and zinc ratios in CZTS stoichiometry were prepared and characterized. The effect of copper and zinc ratio changes were examined on the photocatalytic properties of both NPs and nanocomposite membranes. The photocatalytic effect was tested and BR18 dye was degraded at 88.0% efficiency when exposed to visible light for 180 min in the presence of CZTS1.0 NPs catalyst. The results also exhibited that CZTS NPs blended membrane showed good self-cleaning performance. Second, we investigated the antioxidant, DNA cleavage, and biofilm inhibition activities of CZTS NPs. The antimicrobial activities of CZTS NPs were tested against seven microbial strains. The highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and chelating activities were found as 81.80 +/- 1.75% and 72.19 +/- 4.63%, respectively. CZTS NPs exhibited double-strand DNA cleavage activity at 500 mg/L. They showed significant biofilm inhibition as 92.15 +/- 5.67% against Staphylococcus aureus. CZTS NPs displayed moderate antimicrobial effects against tested microorganisms. Third, CZTS NPs were blended in polyethersulphone (PES) membrane and the morphology as well as performance of the fabricated nanocomposite membranes were systematically investigated by SEM-EDX, porosity, water contact angle, antifouling, and rejection measurements. The water flux of resulting nanocomposite membranes was markedly enhanced due to the introducing of CZTS NPs. Meanwhile, the nanocomposite membranes demonstrated remarkable antifouling properties (flux recovery ratio similar to 94%) in contrast with the bare PES (flux recovery ratio similar to 51%) when BSA was filtrated. The high retention of BSA (100%) as well as high permeation flux (73.8 +/- 9.8 L/m(2)/h) of the CZTS2.00 wt% membrane demonstrated that the constructed nanocomposite membrane possessed the characteristics of a promising membrane for purification of proteins.Öğe Preparation of ZnO nanorods or SiO2 nanoparticles grafted onto basalt ceramic membrane and the use for E. coli removal from water(Elsevier Sci Ltd, 2021) Saleh, Mohammed; Gonca, Serpil; Isik, Zelal; Ozay, Yasin; Harputlu, Ersan; Ozdemir, Sadin; Yalvac, MutluThis article addresses the fabrication of a novel and eco-friendly ceramic membrane based on basalt powder via press and sintering methods with a pore size of 1.5-2 mu m. The basalt ceramic membrane (BCM) was grafted by SiO2 nanoparticles (50-60 nm) and ZnO nanorods (2.2 mu m). The water permeability for the prepared membranes was measured 345.3, 701.4, and 801.9 L/m(2) h bar for bare BCM, SiO2-BCM, and ZnO-BCM, respectively. The prepared membranes were used in Escherichia coli (E. coli) removal, and 100% E. coli removal efficiency was achieved at a transmembrane pressure of 0.5 bar for all membranes. The antimicrobial activities of the solid surfaces for BCM, SiO2-BCM, and ZnO-BCM were also studied using E. coli as a model test microorganisms. The antimicrobial activities for bare BCM, SiO2-BCM, and ZnO-BCM were 20.57%, 74.90%, and 100%, respectively. The results are of great importance in terms of the reusability of membranes and the prevention of biofilm formation in wastewater treatment processes.Öğe Synthesis of Rhombic Dodecahedral Cuprous Oxide Nanoparticles and Investigation of Biological Activity(Springer, 2022) Kucukosman, Ridvan; Isik, Zelal; Ozdemir, Sadin; Gonca, Serpil; Ocakoglu, Kasim; Dizge, NadirThe rhombic dodecahedral cuprous oxide (Cu2O) nanoparticles (NPs) were synthesized with the morphology-controlled one-pot solution-phase technique and several biological activities were investigated. The structural and elemental properties of Cu2O NPs were determined by SEM-EDX and XRD. Antioxidant, antimicrobial, DNA interaction, and biofilm inhibition activities were determined by using DPPH radical scavenging and metal chelating, microdilution, agarose gel electrophoresis, and crystal violet methods, respectively. The newly synthesized copper nanoparticle showed excellent DPPH and iron-chelating activities as 100% at 200 mg/L concentration. It showed good DNA cleavage activity at all concentrations. Cu2O NP had good antibacterial activity against all tested microorganisms. Biofilm inhibition percentage of Cu2O NP was determined as 86.3% at 500 mg/L. The cell viability assay demonstrated that the Cu2O NP showed 100% cell viability inhibition at all concentrations. Generally, owing to the biological active nature of the synthesized copper nanoparticle, these synthesized Cu2O NPs can be used as a therapeutic and antioxidant agent.Öğe Toxicity, Antibacterial, Antioxidant, Antidiabetic, and DNA Cleavage Effects of Dextran-Graft-Polyacrylamide/Zinc Oxide Nanosystems(Springer, 2024) Yalcin, M. Serkan; Ozdemir, Sadin; Prokopiuk, Volodymyr; Virych, Pavlo; Onishchenko, Anatolii; Tollu, Gulsah; Pavlenko, VadimSynthesis of metal oxide nanoparticles-polymer nanocomposites is an emerging strategy in nanotechnology to improve targeted delivery and reduce the toxicity of nanoparticles. In this study, we report biological effects of previously described hybrid nanocomposites containing dextran-graft-polyacrylamide/zinc oxide nanoparticles (D-PAA/ZnO NPs) prepared from zinc sulfate (D-PAA/ZnONPs(SO42-)) and zinc acetate (D-PAA/ZnONPs(-OAc)) focusing primarily on their antimicrobial activity. D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) nanosystems were tested in a complex way to assess their antioxidant activity (DPPH assay), antidiabetic potential (alpha-amylase inhibition), DNA cleavage activity, antimicrobial, and antibiofilm activity. In addition, the toxicity of D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) nanosystems against primary murine splenocytes was tested using MTT assay. The studied nanosystems inhibited E.coli growth. For all the investigated strains, minimum inhibitory concentrations (MICs) of D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) were in the range of 8 mg/L-128 mg/L and 16 mg/L-128 mg/L, respectively. The nanocomposites demonstrated effective antibiofilm properties as 94.27% and 86.43%. The compounds showed good antioxidant, anti-alpha-amylase, and DNA cleavage activities. D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) nanosystems reduced cell viability and promoted cell death of primary murine spleen cells at concentrations higher than those that proved to be antibacterial indicating the presence of therapeutic window. D-PAA/ZnONPs(SO42-) and D-PAA/ZnONPs(-OAc) nanosystems show antioxidant, antidiabetic, DNA cleavage, antimicrobial, and antibiofilm activity against the background of good biocompatibility suggesting the presence of therapeutic potential, which should be further investigated in vivo.
