Gonca, SerpilOzdemir, SadinYefimova, SvetlanaTkachenko, AntonOnishchenko, AnatoliiKlochkov, VladimirKavok, Nataliya2025-03-172025-03-1720210363-90451520-5762https://doi.org/10.1080/03639045.2022.2075007https://hdl.handle.net/20.500.13099/1879Nanotechnology can be applied to design antibacterial agents to combat antibiotic resistance. The aim of the present study was to assess the antimicrobial effects and cytotoxicity of GdYVO4:Eu3+ nanoparticles (NPs). Biofilm inhibition activity, antimicrobial activity, bacterial viability inhibition and DNA cleavage activity of GdYVO4:Eu3+ NPs were studied. In addition, the impact of GdYVO4:Eu3+ NPs on the mitochondrial membrane potential (Delta psi(M)) of host immune cells and, hence, their apoptosis was analyzed by JC-1 staining using flow cytometry. GdYVO4:Eu3+ NPs demonstrated good antimicrobial, cell viability inhibition and DNA cleavage activities. In addition, GdYVO4:Eu3+ NPs showed good biofilm inhibition activity against S. aureus and P. aeruginosa and inhibition percentages were 89.15% and 79.54%, respectively. However, GdYVO4:Eu3+ NPs promoted mitochondrial depolarization and apoptosis of leukocytes at high concentrations. GdYVO4:Eu3+ nanoparticles are promising antibacterial agents. However, more efforts should be exerted to ensure their safety.eninfo:eu-repo/semantics/closedAccessJC-1mitochondrial membrane potentialDNA cleavagebiofilm inhibition activityantimicrobial activitybacterial viabilitynanoparticlesArticle10.1080/03639045.2022.2075007471219661974Q3WOS:0007957375000012-s2.0-8513044817835514217Q1