Acet, OmurNoma, Samir Abbas AliAcet, Burcu OnalDikici, EmrahOsman, BilgenOdabasi, Mehmet2025-03-172025-03-1720230731-70851873-264Xhttps://doi.org/10.1016/j.jpba.2023.115250https://hdl.handle.net/20.500.13099/2041In this study, a L-asparagine (L-Asn) imprinted membranes (L-Asn-MIPs) were synthesized via molecular imprinting for selective and efficient removal of L-Asn. The L-Asn-MIP membrane was prepared by using acrylamide (AAm) and hydroxyethyl methacrylate (HEMA) as a functional monomer and a comonomer, respectively. The membrane was characterized by scanning electron microscopy (SEM) and Fourier Transform infrared spectroscopy (FTIR). The L-Asn adsorption capacity of the membrane was investigated in detail. The maximum L-Asn adsorption capacity was determined as 408.2 mg/g at pH: 7.2, 24 degrees C. Determination of L-Asn binding behaviors of L-Asn-MIPs also shown with Scatchard analyses. The effect of pH on L-Asn adsorption onto the membrane and also the selectivity and reusability of the L-Asn-MIPs for L-Asn adsorption were determined through L-asparaginase (L-ASNase) enzyme activity measurements. The selectivity of the membrane was investigated by using two different ternary mixtures; L-glycine (L-Gly)/L-histidine (L-His)/L-Asn and L-tyrosin (L-Tyr)/L-cystein(L-Cys)/L-Asn. The obtained results showed that the L-Asn-MIP membranes have a high selectivity towards L-Asn.eninfo:eu-repo/semantics/closedAccessAsparagineL-asparaginase activityMolecular imprintingRemovalArticle10.1016/j.jpba.2023.115250226Q2WOS:0009714518000012-s2.0-8514645616236657352Q1