Acet, Omur2025-03-172025-03-1720231011-372X1572-879Xhttps://doi.org/10.1007/s10562-023-04418-8https://hdl.handle.net/20.500.13099/2358L-asparaginase (L-ASNase) is among the important biopharmaceuticals and is utilized in the treatment of acute lymphoblastic. L-ASNase catalyzes its conversion to aspartic acid and ammonia. The use of enzymes loaded on polymeric systems is an alternative way to avoid the stability problem when using enzymes. In addition, drug release applications that can be obtained with loaded enzymes can be very useful, especially in both pharmaceutical and medical implementations, as they can canalize the enzyme to the right site. For this purpose, L-asparaginase loaded smart nanopolymers (ASN-SNPs) were synthesized via miniemulsion process. The SEM, FTIR characterizations and, zeta potential of ASN-SNPs were carried out. ASN-SNPs showed improvement in free enzyme related stability under extreme conditions. On the other hand, the storage stability and reusability of theASN-SNPs were found to be about 63 and 53% of the original activity after 4 weeks days at room temperature and 10 cycles, respectively. The Michaelis-Menten constants (Km) of 6.595 and 1.902 mM, and the maximum reaction rates (Vmax) of 212.766 and 49.02 mu M min(-1) were founded for free and loaded L-ASNase, respectively. The results showed that the designed L-asparaginase loaded SNPs are a promising matrix for their high catalytic efficiency and enhanced stability properties. [GRAPHICS] .eninfo:eu-repo/semantics/closedAccessL-asparaginaseEnzyme activitySmart nanopolymersTemperaturepH sensitive nanopolymerArticle10.1007/s10562-023-04418-81531031743184Q3WOS:0010336327000012-s2.0-85165276960Q2