IMAC application of extracellular polymeric substances doped composite membranes for α-amylase immobilization and kinetic studies

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dc.contributor.authorBani, Nurullah
dc.contributor.authorInanan, Tulden
dc.contributor.authorAcet, Omur
dc.contributor.authorOdabasi, Mehmet
dc.date.accessioned2025-03-17T12:27:02Z
dc.date.available2025-03-17T12:27:02Z
dc.date.issued2024
dc.departmentTarsus Üniversitesi
dc.description.abstractImmobilized metal affinity chromatography (IMAC), extensively utilized technique in affinity chromatography, has proven to be highly effective in purifying alpha-amylase, a crucial enzyme in industrial applications. Here, Poly (2-hydroxyethyl methacrylate) (PHEMA) based composite membranes were fabricated using the radical copolymerization technique in a petri dish. Extracellular polymeric materials (EPS) as the metal binding agent. The attachment of Cu 2+ ions to the membranes occurred through the utilization of EPSs (Cu 2+ -EPS). The membranes having Cu 2+ -EPS (Cu 2+ -EPS-PM) were subjected to various characterization techniques, including scanning electron microscopy (SEM), attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR), swelling tests, and surface area measurements. The investigation also encompassed the examination of various factors, including pH, initial alpha-amylase concentration, temperature, and ionic strength, that exert an influence on the adsorption of alpha-amylase from an aqueous medium. The Cu 2+ -EPS-PMs demonstrated maximum adsorption capacity with a value of 54.2 mg/g polymer (pH 5.0 sodium acetate buffer, temperature: 25 degrees C, initial alpha-amylase concentration of 2 mg/mL). Additionally, it has been observed that the enzyme is able to be used in adsorption and desorption cycles on Cu 2+ -EPS-PM many times. The investigation also focused on examining the impact of pH, temperature, storage, and operational stability over the activities of both free and immobilized alpha-amylase. The outcomes showed that immobilization had positive effects on activity.
dc.identifier.doi10.1016/j.mcat.2024.114198
dc.identifier.issn2468-8231
dc.identifier.scopus2-s2.0-85192531535
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.mcat.2024.114198
dc.identifier.urihttps://hdl.handle.net/20.500.13099/2023
dc.identifier.volume562
dc.identifier.wosWOS:001241387600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofMolecular Catalysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250316
dc.subjectalpha-amylase
dc.subjectMembrane
dc.subjectImmobilized metal affinity chromatography
dc.subjectKinetic activity
dc.subjectExtracellular polymeric materials (EPS)
dc.titleIMAC application of extracellular polymeric substances doped composite membranes for α-amylase immobilization and kinetic studies
dc.typeArticle

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