A New Design to Enhance the Enzyme Activities: Investigation of L-Asparaginase Catalytic Performance by IMAC Effect on g-C3N4 Nanolayers
[ X ]
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Link
Erişim Hakkı
info:eu-repo/semantics/restrictedAccess
Özet
Recently, graphite carbon nitride (g-C3N4) has come to the fore as a new material with its carbon-based two-dimensional structure, simple preparation procedure, and excellent physicochemical stability properties. This study aims to investigate the activity and kinetic studies of the L-asparaginase enzyme via immobilized metal ion affinity chromatography (IMAC) process of g-C3N4 nanolayers. Firstly, g-C3N4 nanolayers were synthetized and Ni2+ ions were binded their surfaces. The synthesized samples were investigated by SEM, ICP-MS, XRD, and FTIR. The highest L-ASNase adsorption on Ni2+-g-C3N4 nanostructures was 444.1 mg/g, at 3 mg/mL L-ASNase concentration. Optimal medium conditions for L-ASNase adsorption occurred at pH 8.0 and 25 °C. The immobilized enzyme showed improved stability relating to the soluble enzyme in extreme situations. On the other hand, the storage stability and reusability of the immobilized enzyme were found to be approximately 64 and 53% of the original activity after 29 days at room temperature and 10 cycles, respectively. From the Michaelis–Menten constants Km and Vmax, both of them decreased after immobilization compare to the free one. The obtained outcomes showed that the g-C3N4 is a suitable matrix for L-asparaginase immobilization with ideal catalytic efficiency and improved stability.
Açıklama
Anahtar Kelimeler
Graphite carbon nitride, Nanolayers, L-ASNase, Enzyme activity, Immobilization
Kaynak
Catalysis Letters
WoS Q Değeri
Q3
Scopus Q Değeri
Cilt
154
Sayı
4
Künye
Sert, B., Acet, Ö., Noma, S.A.A., Osman, B., Odabaşı, M. ve Ocakoğlu, K. (2024). A New Design to Enhance the Enzyme Activities: Investigation of L-Asparaginase Catalytic Performance by IMAC Effect on g-C3N4 Nanolayers. Catal Lett 154 (4), 1547–1557. https://doi.org/10.1007/s10562-023-04405-z
