Immobilization of Alpha-Amylase onto Ni2+ Attached Carbon Felt: Investigation of Kinetic Parameters from Potato Wastewater

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dc.authoridhttps://orcid.org/0000-0003-2807-0425
dc.authoridorcid.org/0000-0002-7805-9315
dc.authorscopusid36088420200
dc.authorwosidDWR-6549-2022
dc.authorwosidCZA-6907-2022
dc.contributor.authorAcet, Ömür
dc.contributor.authorİnanan, Tülden
dc.contributor.authorÖndül Koç, Eda
dc.contributor.authorSert, Buse
dc.contributor.authorÖnal Acet, Burcu
dc.contributor.authorOdabaşı, Mehmet
dc.contributor.authorOcakoğlu, Kasım
dc.contributor.authorDizge, Nadir
dc.date.accessioned2024-08-06T14:43:23Z
dc.date.available2024-08-06T14:43:23Z
dc.date.issued2023
dc.departmentFakülteler, Mühendislik Fakültesi, Mühendislik Temel Bilimleri Bölümü
dc.description.abstract?-amylase is an important enzyme for textile, food, paper, and the pharmaceutical industrial areas. In this study, Ni2+ attached carbon felt structures with nitrogen active site (Ni2+-N-ACF) are produced. The surface morphologies of the N-ACF and Ni2+-N-ACF are investigated by means of scanning electron microscopy (SEM) analysis. Ni2+ ions binding on the N-ACFs are determined by energy dispersive X-ray (EDX) analysis and a graphite furnace atomic absorption spectrometer (AAS). The effect of pH, ionic strength, initial ?-amylase concentration, and temperature parameters is investigated for ?-amylase immobilization on Ni2+-N-ACF structures. In addition, pH and temperature effect on the activities of the free and the immobilized amylase, kinetic parameters, storage, and operational stabilities are made. Lastly, starch degradation in potato waste water is tested on Ni2+-N-ACF. The obtained results show that ?-amylase immobilized Ni2+-N-ACF can be used for starch degradation on an industrial scale.
dc.identifier.citationAcet, Ö., İnanan, T. Ondul Koc, e., Sert, B., Önal Acet, B. Odabaşı, M., Ocakoglu, K. ve Dizge, N. (2023). Immobilization of Alpha-Amylase onto Ni2+ Attached Carbon Felt: Investigation of Kinetic Parameters from Potato Wastewater. Starch, 75 (11-12). Wiley
dc.identifier.doi10.1002/star.202300033
dc.identifier.endpage8en_US
dc.identifier.issue11-12en_US
dc.identifier.scopus2-s2.0-85168586502
dc.identifier.scopusqualityN/A
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1002/star.202300033
dc.identifier.urihttps://hdl.handle.net/20.500.13099/332
dc.identifier.volume75en_US
dc.identifier.wosWOS:001115819400016
dc.identifier.wosqualityQ2
dc.indekslendigikaynakScopus
dc.institutionauthorAcet, Ömür
dc.institutionauthorSert, Buse
dc.institutionauthorOcakoğlu, Kasım
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofStarch
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/embargoedAccess
dc.subjectAlpha-Amylase
dc.subjectatomic absorption spectrometer (AAS)
dc.titleImmobilization of Alpha-Amylase onto Ni2+ Attached Carbon Felt: Investigation of Kinetic Parameters from Potato Wastewater
dc.typeArticle

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