Immobilization of Alpha-Amylase onto Ni2+ Attached Carbon Felt: Investigation of Kinetic Parameters from Potato Wastewater

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dc.authoridhttps://orcid.org/0000-0003-2807-0425en_US
dc.authoridorcid.org/0000-0002-7805-9315en_US
dc.authorscopusid36088420200en_US
dc.authorwosidDWR-6549-2022en_US
dc.authorwosidCZA-6907-2022en_US
dc.contributor.authorAcet, Ömür
dc.contributor.authorİnanan, Tülden
dc.contributor.authorÖndül Koç, Eda
dc.contributor.authorSert, Buse
dc.contributor.authorÖnal Acet, Burcu
dc.contributor.authorOdabaşı, Mehmet
dc.contributor.authorOcakoğlu, Kasım
dc.contributor.authorDizge, Nadir
dc.date.accessioned2024-08-06T14:43:23Z
dc.date.available2024-08-06T14:43:23Z
dc.date.issued2023en_US
dc.departmentFakülteler, Mühendislik Fakültesi, Mühendislik Temel Bilimleri Bölümüen_US
dc.description.abstractα-amylase is an important enzyme for textile, food, paper, and the pharmaceutical industrial areas. In this study, Ni2+ attached carbon felt structures with nitrogen active site (Ni2+-N-ACF) are produced. The surface morphologies of the N-ACF and Ni2+-N-ACF are investigated by means of scanning electron microscopy (SEM) analysis. Ni2+ ions binding on the N-ACFs are determined by energy dispersive X-ray (EDX) analysis and a graphite furnace atomic absorption spectrometer (AAS). The effect of pH, ionic strength, initial α-amylase concentration, and temperature parameters is investigated for α-amylase immobilization on Ni2+-N-ACF structures. In addition, pH and temperature effect on the activities of the free and the immobilized amylase, kinetic parameters, storage, and operational stabilities are made. Lastly, starch degradation in potato waste water is tested on Ni2+-N-ACF. The obtained results show that α-amylase immobilized Ni2+-N-ACF can be used for starch degradation on an industrial scale.en_US
dc.identifier.citationAcet, Ö., İnanan, T. Ondul Koc, e., Sert, B., Önal Acet, B. Odabaşı, M., Ocakoglu, K. ve Dizge, N. (2023). Immobilization of Alpha-Amylase onto Ni2+ Attached Carbon Felt: Investigation of Kinetic Parameters from Potato Wastewater. Starch, 75 (11-12). Wileyen_US
dc.identifier.doi10.1002/star.202300033en_US
dc.identifier.endpage8en_US
dc.identifier.issue11-12en_US
dc.identifier.scopus2-s2.0-85168586502en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1002/star.202300033
dc.identifier.urihttps://hdl.handle.net/20.500.13099/332
dc.identifier.volume75en_US
dc.identifier.wosqualityQ2en_US
dc.institutionauthorAcet, Ömür
dc.institutionauthorSert, Buse
dc.institutionauthorOcakoğlu, Kasım
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.ispartofStarchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectAlpha-Amylaseen_US
dc.subjectatomic absorption spectrometer (AAS)en_US
dc.titleImmobilization of Alpha-Amylase onto Ni2+ Attached Carbon Felt: Investigation of Kinetic Parameters from Potato Wastewateren_US
dc.typearticleen_US

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