First protein affinity application of Cu2+-bound pure inorganic nanoflowers

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dc.authoridShcharbin, Dzmitry/0000-0002-1414-4465
dc.authoridOZDEMIR, NALAN/0000-0002-8930-5198
dc.authoridODABASI, Mehmet/0000-0002-3288-132X
dc.authorid, Inessa/0000-0003-2614-9914
dc.authoridAcet, Omur/0000-0003-1864-5694
dc.authoridONAL ACET, Burcu/0000-0003-2408-8660
dc.authoridDzmitruk, Volha/0000-0002-2591-3346
dc.contributor.authorOnal, Burcu
dc.contributor.authorAcet, Omur
dc.contributor.authorDzmitruk, Volha
dc.contributor.authorHalets-Bui, Inessa
dc.contributor.authorShcharbin, Dzmitry
dc.contributor.authorOzdemir, Nalan
dc.contributor.authorOdabasi, Mehmet
dc.date.accessioned2025-03-17T12:27:38Z
dc.date.available2025-03-17T12:27:38Z
dc.date.issued2022
dc.departmentTarsus Üniversitesi
dc.description.abstractToday, a new kind of materials is introduced to separation media day by day to increase the efficiency of the separation processes, and multiple-petalled nanostructured materials are one of them. In this study, new pure inorganic copper phosphate nanoflowers (pCP-NFs) were synthesized, and some environmental conditions affecting on binding mechanism with human serum albumin were evaluated via changing medium pH, temperature, initial human serum albumin (HSA) amount and salt concentrations. Before experimental studies, pCP-NFs were subjected to some characterization tests such as scanning electron microscopy, energy-dispersive X-ray, X-ray diffraction and Fourier transform infrared spectroscopy. Besides a lot of valuable instrumental data, some obtained experimental ones as follows: after Cu2+ ions attachment to pCP-NFs as ligand, maximum HSA adsorption capacity of obtained Cu2+-pCP-NFs was found as 225.7 mg/g with an initial concentration of 1.5 mg/mL at pH 7 and 25 degrees C. Langmuir and Freundlich adsorption equations were evaluated for determination of appropriate adsorption model in interaction, and Langmuir model found as the fittest one with a R-2 of 0.9949 was also reviewed to determine Gibbs free energy between HSA and Cu2+-pCP-NFs interaction.
dc.description.sponsorshipScientific and Technological Research Council of Turkey [118Z037]; Belarusian Republican Foundation for Fundamental Research [B18TUB-001]
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey (Grant Number:118Z037) and by Belarusian Republican Foundation for Fundamental Research, Grant B18TUB-001. Authors gratefully acknowledge use of the services and facilities of Technology Research and Implementation Center of Erciyes University (TAUM) and Scientific and Technological Application and Research Center of Aksaray University (ASUBTAM).
dc.identifier.doi10.1007/s00289-021-03557-5
dc.identifier.endpage3251
dc.identifier.issn0170-0839
dc.identifier.issn1436-2449
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85103233990
dc.identifier.scopusqualityQ1
dc.identifier.startpage3233
dc.identifier.urihttps://doi.org/10.1007/s00289-021-03557-5
dc.identifier.urihttps://hdl.handle.net/20.500.13099/2371
dc.identifier.volume79
dc.identifier.wosWOS:000631742700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPolymer Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250316
dc.subjectPure copper phosphate nanoflowers
dc.subjectImmobilized metal affinity chromatography
dc.subjectProtein separation
dc.subjectInorganic sorbents
dc.titleFirst protein affinity application of Cu2+-bound pure inorganic nanoflowers
dc.typeArticle

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