In Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis-Associated Genes

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dc.contributor.authorYunusoglu, Oruc
dc.contributor.authorTürkmen, Ömer
dc.contributor.authorBerkoz, Mehmet
dc.contributor.authorYıldırım, Metin
dc.contributor.authorYalın, Serap
dc.date.accessioned2025-03-17T12:22:56Z
dc.date.available2025-03-17T12:22:56Z
dc.date.issued2022
dc.departmentTarsus Üniversitesi
dc.description.abstractIn recent years, obesity has been associated with heart diseases, type 2 diabetes, obstructive sleep apnea, certain types of cancer and osteoarthritis, and become a global health problem. Therefore, researchers seek to find functional drugs against obesity. For the past few decades, medicinal plants have been examined to for their anti-obesity effects, including Aloe vera. In this study, it was aimed to elucidate the inhibitory effect of Aloe vera extract on adipogenesis. Firstly, 3T3-L1 pre-adipocytes were stimulated so as to differentiate into mature adipocyte using adipogenic differentiation cocktail consisting of 10 μg/mL insulin, 0.5 mM isobutylmethylxanthine, 0.25 mM dexamethasone, and 100 ng/mL biotin on day 0. Various concentrations (10-50 µg/mL) of Aloe vera extract with no cytotoxic effect were applied to differentiated 3T3-L1 cells. Glyceraldehyde-3-phosphate dehydrogenase (GPDH) activity, Oil r ed O staining, intracellular triglyceride levels, and gene expressions of transcription factors and lipolysis-associated genes were examined in order to investigate the effect of Aloe vera extract on adipocyte differentiation. Aloe vera treatment caused a continuous decrease in cell size and intracellular triglyceride accumulation. Despite the fact that GPDH activity, mRNA levels of transcription factors and lipolysis-associated genes decreased in mature adipocytes treated with Aloe vera extract. These results suggest that Aloe vera may have a therapeutic effect in prevention and/or treatment of adipogenesis-related obesity. © 2022, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
dc.description.sponsorshipOffice of Scientific Research Projects of Van Yuzuncu Yil University, (THD-2016-5428)
dc.identifier.doi10.5505/ejm.2022.13285
dc.identifier.endpage528
dc.identifier.issn1301-0883
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85140013227
dc.identifier.scopusqualityQ4
dc.identifier.startpage519
dc.identifier.trdizinid1175518
dc.identifier.urihttps://doi.org/10.5505/ejm.2022.13285
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1175518
dc.identifier.urihttps://hdl.handle.net/20.500.13099/1491
dc.identifier.volume27
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherYuzuncu Yil Universitesi Tip Fakultesi
dc.relation.ispartofEastern Journal of Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20250316
dc.subject3T3-L1
dc.subjectadipogenesis
dc.subjectAloe vera
dc.subjectcell differentiation
dc.subjectlipolysis-associated genes
dc.subjecttranscription factors
dc.titleIn Vitro Anti-Obesity Effect of Aloe vera Extract Through Transcription Factors and Lipolysis-Associated Genes
dc.typeArticle

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