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    Exploration of Agrocybe aegerita mushroom polysaccharide-polyphenolic complex: Functional properties, binding efficiency, and biological activities
    (Elsevier, 2025) Bains, Aarti; Goksen, Gulden; Ali, Nemat; Khan, Mohammad Rashid; Patil, Sandip; Chawla, Prince
    This study investigates the functional and biological activities of a polysaccharide-polyphenolic complex derived from the edible mushroom Agrocybe aegerita. Polyphenols were extracted using a modified solvent evaporation technique, and polysaccharides (AMP) were extracted using enzyme-assisted methods, yielding 8.02 %. The presence of fructose, mannose, glucose, galactose, sucrose, and maltose in varying amounts was confirmed. Different mushroom extracts concentrations (0.025-1.00 %) were tested for interaction with AMP. Samples with 0.2 % and 0.5 % extracts showed significantly higher binding efficiency with polysaccharides. AMP exhibited a particle size of 319 nm, while mushroom polysaccharide-polyphenolic compound complex (AMPP) revealed 136 nm with an irregular shape and smooth surface. Both AMP and AMPP showed three stages of decomposition, with distinct weight loss. Anti-quorum sensing tests against P. aeruginosa PAO1 showed that AMPP significantly decreased pyocyanin, pyoverdine, and swarming activity and exhibited higher biofilm inhibition. These findings suggest that the AMPP has substantial potential for developing sustainable health products, owing to its enhanced bioactivity.
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    Influence of dual succinylation and ultrasonication modification on the amino acid content, structural and functional properties of Chickpea (Cicer arietinum L.) protein concentrate
    (Elsevier Sci Ltd, 2024) Patil, Nikhil Dnyaneshwar; Bains, Aarti; Kaur, Sawinder; Yadav, Rahul; Ali, Nemat; Patil, Sandip; Goksen, Gulden
    Chickpea protein, a valuable plant -based source, offers versatile applications, yet the impact of modifications like succinylation and ultrasonication on its properties remains unclear. This study explored dual succinylation and ultrasonication modification to enhance its functionality and application. Modified chickpea protein with a degree of succinylation of 96.75 %, showed enhanced water holding capacity 39.83 %, oil holding capacity 54.02 %, solubility 7.20 %, and emulsifying capacity 23.17 %, compared to native protein. Despite reduced amino acid content (64.50 %), particularly lysine, succinylation increased sulfhydryl by 1.74 %, reducing hydrophobicity (Ho) by 41.87 % and causing structural changes. Ultrasonication further reduced particle size by 82.57 % and increased zeta potential and amino acid content (57.47 %). The dual-modified protein exhibited a non-significant increase in antimicrobial activity against Staphylococcus aureus (25.93 +/- 1.36 mm) compared to the native protein (25.28 +/- 1.05 mm). In conclusion, succinylation combined with ultrasonication offers a promising strategy to enhance chickpea protein's physicochemical properties for diverse applications.
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    Öğe
    Synthesis of Berberis aristate rhizome extract stabilized magnesium nanoparticles using green chemistry: rhizome characterization, in vitro antimicrobial and anti-inflammatory activity
    (Taylor & Francis Ltd, 2024) Teja, Talla Sasi; Patil, Sandip; Chawla, Prince; Bains, Aarti; Goksen, Gulden; Ali, Nemat; AlAsmari, Abdullah F.
    In the present study, magnesium nanoparticles (Mg NPs) were synthesized utilizing an aqueous extract of Berberis aristate rhizome and evaluated for antimicrobial and anti-inflammatory activity. Technofunctional properties of rhizome powder were evaluated and during thermal stability evaluation four stages of decomposition with a maximum delta Y value of 76.04 % was observed. Optimization of Mg NPs was carried out by employing eight different concentrations (C1-C8) and the C4 showed maximum absorbance at 330 nm confirming the NPs synthesis. The Mg NPs showed the particle size of 62 nm, zeta potential of -24.7 mV and hexagonal mprphology. Potential inhibition against S. aureus and E. coli (76.78 +/- 0.05% and 74.62 +/- 0.17%)and anti-inflammatory activity ranging from 42.43 +/- 0.07-82.92 +/- 0.04% was observed for Mg NPs. Therefore, green synthesis of Mg NPs is a promising approach for the development ofbiological active NPs to cure microbial infections.