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Öğe A rational approach for 3D recognition and removal of L-asparagine via molecularly imprinted membranes(Elsevier, 2023) Acet, Omur; Noma, Samir Abbas Ali; Acet, Burcu Onal; Dikici, Emrah; Osman, Bilgen; Odabasi, MehmetIn this study, a L-asparagine (L-Asn) imprinted membranes (L-Asn-MIPs) were synthesized via molecular imprinting for selective and efficient removal of L-Asn. The L-Asn-MIP membrane was prepared by using acrylamide (AAm) and hydroxyethyl methacrylate (HEMA) as a functional monomer and a comonomer, respectively. The membrane was characterized by scanning electron microscopy (SEM) and Fourier Transform infrared spectroscopy (FTIR). The L-Asn adsorption capacity of the membrane was investigated in detail. The maximum L-Asn adsorption capacity was determined as 408.2 mg/g at pH: 7.2, 24 degrees C. Determination of L-Asn binding behaviors of L-Asn-MIPs also shown with Scatchard analyses. The effect of pH on L-Asn adsorption onto the membrane and also the selectivity and reusability of the L-Asn-MIPs for L-Asn adsorption were determined through L-asparaginase (L-ASNase) enzyme activity measurements. The selectivity of the membrane was investigated by using two different ternary mixtures; L-glycine (L-Gly)/L-histidine (L-His)/L-Asn and L-tyrosin (L-Tyr)/L-cystein(L-Cys)/L-Asn. The obtained results showed that the L-Asn-MIP membranes have a high selectivity towards L-Asn.Öğe A Review for Uncovering the Protein-Nanoparticle Alliance: Implications of the Protein Corona for Biomedical Applications(Mdpi, 2024) Onal Acet, Burcu; Gul, Desiree; Stauber, Roland H.; Odabasi, Mehmet; Acet, OmurUnderstanding both the physicochemical and biological interactions of nanoparticles is mandatory for the biomedical application of nanomaterials. By binding proteins, nanoparticles acquire new surface identities in biological fluids, the protein corona. Various studies have revealed the dynamic structure and nano-bio interactions of the protein corona. The binding of proteins not only imparts new surface identities to nanoparticles in biological fluids but also significantly influences their bioactivity, stability, and targeting specificity. Interestingly, recent endeavors have been undertaken to harness the potential of the protein corona instead of evading its presence. Exploitation of this 'protein-nanoparticle alliance' has significant potential to change the field of nanomedicine. Here, we present a thorough examination of the latest research on protein corona, encompassing its formation, dynamics, recent developments, and diverse bioapplications. Furthermore, we also aim to explore the interactions at the nano-bio interface, paving the way for innovative strategies to advance the application potential of the protein corona. By addressing challenges and promises in controlling protein corona formation, this review provides insights into the evolving landscape of the 'protein-nanoparticle alliance' and highlights emerging.Öğe Anti-cancer activity of naringenin loaded smart polymeric nanoparticles in breast cancer(Elsevier, 2022) Yildirim, Metin; Acet, Omur; Yetkin, Derya; Acet, Burcu Onal; Karakoc, Veyis; Odabasi, MehmetBreast cancer is the most common form of cancer among women worldwide, and approximately comprise 25% of all female malignancies. Naringenin (Nar) is a promising anticancer agent for breast cancer. However, its use as a therapeutic agent is limited due to its poor water solubility and bioavailability. The purpose of the present study is to prepare pH and thermo sensitive smart polymeric nanoparticles carrying naringenin (NarSPNPs) to improve bioavailability, and increase therapeutic efficacy against breast cancer. N-isopropylacrylamide and Vinyl imidazole were used as thermo and pH sensitive monomers, respectively. NarSPNPs were characterized using dynamic light scattering (DLS) analyses, SEM and FTIR for particle size and potential analysis, surface morphology and functional group determinations, respectively. Release profile and its effects on cell proliferation, apoptosis and cell cycle in breast cancer were also studied. Physicochemical characterization of newly prepared NarSPNPs, cytotoxicity, and IC50 assessments confirmed their stability and bioactivity as an anti-breast cancer agent with no toxicity against human epithelia cells. These findings together with flow cytometry analysis, revealed that apoptosis is the main mechanism underlying cell death after NarSPNPs treatment.Öğe Detection of N-hexanoyl-L-homoserine lactone via MIP-based QCM sensor: preparation and characterization(Springer, 2023) Acet, Omur; Odabasi, MehmetWhile high living organisms use sounds and words to communicate with each other, bacteria provide this function with some communication molecules. These communication molecules are expressed as Quorum Sensing (QS) or chemicals produced by the environment sensing system and used by bacteria to communicate. This case revealed that bacteria are talking. N-acyl-homoserine-lactones (AHL), which are the main signaling molecules of gram-negative bacteria, may express the pathogenic factors, and Quorum Sensing (QS) system may play an important role in the identification of the being virus. AHLs are produced at low concentrations that are difficult to detect with general techniques. In this study, molecularly imprinted polymeric membranes, which are specific to lactone with 6-carbons (C6-HSL) were prepared on QCM chips, and molecularly imprinted QCM chips were utilized to detect N-hexanoyl-L-homoserine lactone (C6-HSL) with high sensitivity from the aqueous medium. Characterization of the synthesized polymers were analyzed by AFM, SEM, contact angle and FTIR-ATR spectrophotometer analyzes. The maximum C6-HSL adsorption on the C6-HSL imprinted QCM chip surface was observed at 1000 ng/mL concentration, pH 7.0. Moreover, GC-MS results correlated with the results of our studies, and they confirmed the sensitivity of synthesized polymers toward C6-HSL in solution containing competitor molecules.Öğe Enhanced Anti-Cancer Efficacy of Hesperidin through Smart Polymeric Nanoparticles Targeting Prostate Cancer(Wiley-V C H Verlag Gmbh, 2024) Yildirim, Metin; Acet, Omur; Acet, Burcu Onal; Karakoc, Veyis; Odabasi, MehmetThe rapid progress of various nanotechnology tools is currently being utilized in the management of several fatal illnesses, including cancer. Nanopolymers that encapsulate anti-cancer medication present a highly encouraging substitute to traditional therapies, primarily because of their specific targeting and accurate functionality, making them suitable for a variety of uses. Poly(2-hydroxyethyl methacrylate) (pHEMA) is a non-toxic polymer derived from the monomer HEMA, which is known for its toxicity. It can be combined with various other polymers and is associated with minimal immune response. The objective of the current investigation is to produce smart polymeric nanoparticles that are responsive to changes in pH and temperature, and capable of encapsulating hesperidin. These hesperidin-loaded poly(2-Hydroxyethyl methacrylate-N-isopropylacrylamide-Vinyl imidazole) nanoparticles, referred to as HesSNPs, aim to enhance the bioavailability of hesperidin and augment its therapeutic effectiveness in combating prostate cancer. N-isopropylacrylamide and Vinyl imidazole were employed as monomers sensitive to temperature and pH, respectively. Nanopolymers that were synthesized underwent characterization through dynamic light scattering (DLS) analyses, scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR) to analyze particle size and charge, surface morphology, and functional group determinations, respectively. HesSNPs decreased cell viability of DU-145 cells. Flow cytometry analysis revealed that apoptosis is the main mechanism underlying cell death after HesSNPs treatment. Smart polymeric nanoparticles (HesSNPs), designed to encapsulate hesperidin, are synthesized from pH and temperature-responsive monomers. Characterized by DLS, SEM, and FTIR, these nanoparticles enhance hesperidin bioavailability. HesSNPs reduce prostate cancer cell viability (DU-145), with flow cytometry indicating apoptosis as the primary mechanism. This study highlights the potential of HesSNPs in advancing cancer treatment strategies. imageÖğe First protein affinity application of Cu2+-bound pure inorganic nanoflowers(Springer, 2022) Onal, Burcu; Acet, Omur; Dzmitruk, Volha; Halets-Bui, Inessa; Shcharbin, Dzmitry; Ozdemir, Nalan; Odabasi, MehmetToday, a new kind of materials is introduced to separation media day by day to increase the efficiency of the separation processes, and multiple-petalled nanostructured materials are one of them. In this study, new pure inorganic copper phosphate nanoflowers (pCP-NFs) were synthesized, and some environmental conditions affecting on binding mechanism with human serum albumin were evaluated via changing medium pH, temperature, initial human serum albumin (HSA) amount and salt concentrations. Before experimental studies, pCP-NFs were subjected to some characterization tests such as scanning electron microscopy, energy-dispersive X-ray, X-ray diffraction and Fourier transform infrared spectroscopy. Besides a lot of valuable instrumental data, some obtained experimental ones as follows: after Cu2+ ions attachment to pCP-NFs as ligand, maximum HSA adsorption capacity of obtained Cu2+-pCP-NFs was found as 225.7 mg/g with an initial concentration of 1.5 mg/mL at pH 7 and 25 degrees C. Langmuir and Freundlich adsorption equations were evaluated for determination of appropriate adsorption model in interaction, and Langmuir model found as the fittest one with a R-2 of 0.9949 was also reviewed to determine Gibbs free energy between HSA and Cu2+-pCP-NFs interaction.Öğe IMAC application of extracellular polymeric substances doped composite membranes for α-amylase immobilization and kinetic studies(Elsevier, 2024) Bani, Nurullah; Inanan, Tulden; Acet, Omur; Odabasi, MehmetImmobilized metal affinity chromatography (IMAC), extensively utilized technique in affinity chromatography, has proven to be highly effective in purifying alpha-amylase, a crucial enzyme in industrial applications. Here, Poly (2-hydroxyethyl methacrylate) (PHEMA) based composite membranes were fabricated using the radical copolymerization technique in a petri dish. Extracellular polymeric materials (EPS) as the metal binding agent. The attachment of Cu 2+ ions to the membranes occurred through the utilization of EPSs (Cu 2+ -EPS). The membranes having Cu 2+ -EPS (Cu 2+ -EPS-PM) were subjected to various characterization techniques, including scanning electron microscopy (SEM), attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR), swelling tests, and surface area measurements. The investigation also encompassed the examination of various factors, including pH, initial alpha-amylase concentration, temperature, and ionic strength, that exert an influence on the adsorption of alpha-amylase from an aqueous medium. The Cu 2+ -EPS-PMs demonstrated maximum adsorption capacity with a value of 54.2 mg/g polymer (pH 5.0 sodium acetate buffer, temperature: 25 degrees C, initial alpha-amylase concentration of 2 mg/mL). Additionally, it has been observed that the enzyme is able to be used in adsorption and desorption cycles on Cu 2+ -EPS-PM many times. The investigation also focused on examining the impact of pH, temperature, storage, and operational stability over the activities of both free and immobilized alpha-amylase. The outcomes showed that immobilization had positive effects on activity.Öğe Inhibition of bacterial adhesion by epigallocatechin gallate attached polymeric membranes(Elsevier, 2023) Acet, Omur; Dikici, Emrah; Acet, Burcu Onal; Odabasi, Mehmet; Mijakovic, Ivan; Pandit, SantoshMicrobial adhesion and formation of biofilms cause a serious problem in several areas including but not limited to food spoilage, industrial corrosion and nosocomial infections. These microbial biofilms pose a serious threat to human health since microbial communities in the biofilm matrix are protected with exopolymeric substances and difficult to eradicate with antibiotics. Hence, the prevention of microbial adhesion followed by biofilm formation is one of the promising strategies to prevent these consequences. The attachment of antimicrobial agents, coatings of nanomaterials and synthesis of hybrid materials are widely used approach to develop surfaces having potential to hinder bacterial adhesion and biofilm formation. In this study, epigallocatechin gallate (EGCG) is attached on p(HEMA-co-GMA) membranes to prevent the bacterial colonization. The attachment of EGCG to membranes was proved by Fourier-transform infrared spectroscopy (FT-IR). The synthesized membrane showed porous structure (SEM), and desirable swelling degree, which are ideal when it comes to the application in biotechnology and biomedicine. Furthermore, EGCG attached membrane showed significant potential to prevent the microbial colonization on the surface. The obtained results suggest that EGCG attached polymer could be used as an alternative approach to prevent the microbial colonization on the biomedical surfaces, food processing equipment as well as development of microbial resistant food packaging systems.Öğe L-asparaginase immobilized p(HEMA-GMA) cryogels: A recent study for biochemical, thermodynamic and kinetic parameters(Elsevier Sci Ltd, 2021) Noma, Samir Abbas Ali; Acet, Omur; Ulu, Ahmet; Onal, Burcu; Odabasi, Mehmet; Ates, BurhanCryogels have recently been attracted intense attention as suitable carriers for enzyme immobilization. Herein, L-asparaginase was selected as the model enzyme due to its application such as pharmaceutical and food. Under optimum conditions, L-asparaginase was immobilized on poly (2-hydroxyethyl methacrylate-glycidyl methacrylate) cryogels with 68.8% of immobilization yield and 69.3% of activity recovery. The immobilized enzyme exhibited improved stability with respect to the soluble enzyme at extreme conditions, especially around acidic pH and high temperature. Also, the storage stability and reusability of the immobilized enzyme were found to be approximately 54% and 52% of the original activity after 28 days at room temperature and 10 cycles, respectively. The thermodynamic studies indicated that activation energy (E-a) of the free enzyme decreased from 13.08 to 10.97 kJ/mol, which means an increase in the thermostability of L-asparaginase. The Michaelis-Menten constants (K-m) of 2.04 and 1.67 mM, and the maximum reaction rates (V-max) of 170.0 and 115.0 mu M min(-1) were estimated for soluble and immobilized L-asparaginase, respectively. These findings demonstrated that the designed cryogels turn out to be a good carrier matrix for L-asparaginase immobilization with high catalytic efficiency and enhanced stability.Öğe ?Lab-on-pol? colormatic sensor platforms: Melamine detection with color change on melamine imprinted membranes(Elsevier, 2023) Dikici, Emrah; Acet, Burcu Onal; Acet, Omur; Odabasi, MehmetThere is an urgent need to improve a specific, equipment-free and user friendly technique for detecting melamine in food samples. Here, melamine imprinted polymeric membranes were synthesized, and tested for selectivity in aqueous solutions against cyromazine used as competitor agent, and real milk samples spiked with melamine. Scanning electron microscope, atomic force microscopy, energy-dispersive X-ray and optical profilometer devices were used for characterization. Fe+3 ions were used as marker to obtain color change based on melamine concentration. The color intensity on MIP membranes was monitored by means of a color analysis application (Image J software) via a smartphone. Some obtained results as fallows: Melamine binding to 3D cavities of membrane came true in about 20 min. The linear regression plot showed a correlation coefficient (R2) of 0.995 in the range of 10 mu M-50 mu M according to Beer Lambert's law. A low concentration of melamine, (e.g., 10 mu M) was determined in raw milk. Melamine imprinted polymeric membranes showed 7.575 times more sensitivity for melamine than cyromazine. Limit of detection (LOD) and limit of quantification (LOQ) were computed as 9.9 mu M and 30.2 mu M, respectively. Here, a new kind of smart polymer was designed, and successfully applied for the easy, portable and on-site colorimetric analysis via imprinted polymers, and named as lab-on-polsystem. HPLC analysis method was also used to validate this new analysis method.Öğe Molecular imprinted polymers: important advances in biochemistry, biomedical and biotechnology(Springer, 2024) Acet, Burcu Onal; Inanan, Tulden; Salieva, Kalipa; Borkoev, Bakyt; Odabasi, Mehmet; Acet, OmurMolecularly imprinted polymers (MIPs) are handmade receptors that mimic the binding of natural antibodies. In other words, MIPs can selectively bind to the target molecule and qualify as bio-inspired synthetic materials. Today, MIPs are used extensively and are being developed further for biological applications. High cost and time consuming techniques are compelling factors for the field of biochemistry, biomedicine and biotechnology (3B), and there is an urgent need for an alternative, cheap, easy to produce, fast and effective method in these fields. MIPs stand out as a promising way for this purpose. MIPs have superiorities such as specific recognition specificity, excellent sensitivity selectivity and reusability. From this point of view, we examined MIP-related drug delivery studies, cell recognition, enzyme applications, in vivo applications, and applications for some important biomolecules. The aim of this review is to compile the utilizations, advantages, important developments and future expectations of MIPs for the fields of 3B.Öğe N-acyl homoserine lactone molecules assisted quorum sensing: effects consequences and monitoring of bacteria talking in real life(Springer, 2021) Acet, Omur; Erdonmez, Demet; Acet, Burcu Onal; Odabasi, MehmetBacteria utilize small signal molecules to monitor population densities. Bacteria arrange gene regulation in a method called Quorum Sensing (QS). The most widespread signalling molecules are N-Acyl Homoserine Lactones (AHLs/HSLs) for Gram-negative bacteria communities. QS plays significant role in the organizing of the bacterial gene that adapts to harsh environmental conditions for bacteria. It is involved in the arrangement of duties, such as biofilm formation occurrence, virulence activity of bacteria, production of antibiotics, plasmid conjugal transfer incident, pigmentation phenomenon and production of exopolysaccharide (EPS). QS obviously impacts on human health, agriculture and environment. AHL-related QS researches have been extensively studied and understood in depth for cell to cell intercommunication channel in Gram-negative bacteria. It is understood that AHL-based QS research has been extensively studied for cell-to-cell communication in Gram-negative bacteria; hence, a comprehensive study of AHLs, which are bacterial signal molecules, is required. The purpose of this review is to examine the effects of QS-mediated AHLs in many areas by looking at them from a different perspectives, such as clinic samples, food industry, aquatic life and wastewater treatment system.Öğe Revolution in Cancer Treatment: How Are Intelligently Designed Nanostructures Changing the Game?(Mdpi, 2024) Guel, Desiree; Acet, Burcu Oal; Lu, Qiang; Stauber, Roland H.; Odabasi, Mehmet; Acet, OmurNanoparticles (NPs) are extremely important tools to overcome the limitations imposed by therapeutic agents and effectively overcome biological barriers. Smart designed/tuned nanostructures can be extremely effective for cancer treatment. The selection and design of nanostructures and the adjustment of size and surface properties are extremely important, especially for some precision treatments and drug delivery (DD). By designing specific methods, an important era can be opened in the biomedical field for personalized and precise treatment. Here, we focus on advances in the selection and design of nanostructures, as well as on how the structure and shape, size, charge, and surface properties of nanostructures in biological fluids (BFs) can be affected. We discussed the applications of specialized nanostructures in the therapy of head and neck cancer (HNC), which is a difficult and aggressive type of cancer to treat, to give an impetus for novel treatment approaches in this field. We also comprehensively touched on the shortcomings, current trends, and future perspectives when using nanostructures in the treatment of cancer.Öğe Synthesis, characterization and anticancer effect of doxorubicin-loaded dual stimuli-responsive smart nanopolymers(Beilstein-Institut, 2024) Acet, Omur; Kirsanov, Pavel; Acet, Burcu Onal; Halets-Bui, Inessa; Shcharbin, Dzmitry; Comert, Seyda Ceylan; Odabasi, MehmetNanopolymers represent a significant group of delivery vehicles for hydrophobic drugs. In particular, dual stimuli-responsive smart polymer nanomaterials might be extremely useful for drug delivery and release. We analyzed the possibility to include the known antitumor drug doxorubicin (DOX), which has antimitotic and antiproliferative effects, in a nanopolymer complex. Thus, doxorubicin-loaded temperature- and pH-sensitive smart nanopolymers (DOX-SNPs) were produced. Characterizations of the synthesized nanostructures were carried out including zeta potential measurements, Fourier-transform infrared spectroscopy, and scanning electron microscopy. The loading capacity of the nanopolymers for DOX was investigated, and encapsulation and release studies were carried out. In a final step, the cytotoxicity of the DOX-nanopolymer complexes against the HeLa cancer cell line at different concentrations and incubation times was studied. The DOX release depended on temperature and pH value of the release medium, with the highest release at pH 6.0 and 41 degrees C. This effect was similar to that observed for the commercial liposomal formulation of doxorubicin Doxil. The obtained results demonstrated that smart nanopolymers can be efficiently used to create new types of doxorubicin-based drugs.Öğe Synthesis, Characterization, and Investigation of Corona Formation of Dipeptide-Based Nanomaterials(Mdpi, 2025) Dikici, Emrah; Acet, Burcu Onal; Bozdogan, Betuel; Acet, Omur; Halets-Bui, Inessa; Shcharbin, Dzmitry; Odabasi, MehmetPeptide-based nanomaterials can be easily functionalized due to their functional groups, as well as being biocompatible, stable under physiological conditions, and nontoxic. Here, diphenylalanineamide-based nanomaterials (FFANMs) were synthesized, decorated with Ca2+ ions to set the surface charge, and characterized for possible use in gene delivery and drug release studies. FFANMs were characterized by SEM, TEM, dynamic light scattering (DLS), and LC-MS/MS. Corona formation and biocompatible studies were also carried out. Some of the data obtained are as follows: FFANMs have a diameter of approximately 87.93 nm. While the zeta potentials of FFANMs and Ca2+@FFANMs were -20.1 mV and +9.3 mV, respectively, after corona formation with HSA and IgG proteins, they were shifted to -7.6 mV and -3.7 mV, respectively. For gene delivery studies, zeta potentials of Ca2+@FFANMs and DNA interactions were also studied and found to shift to -9.7 mV. Cytotoxicity and biocompatibility studies of NMs were also studied on HeLa and HT29 cell lines, and decreases of about 5% and 10% in viability at the end of 24 h and 72 h incubation times were found. We think that the results obtained from this study will assist the groups working in the relevant field.Öğe Things to Know and Latest Trends in the Design and Application of Nanoplatforms in Cancer Treatment(Springer, 2024) Yildirim, Metin; Acet, Burcu Onal; Dikici, Emrah; Odabasi, Mehmet; Acet, OmurNanotechnology has a crucial potential in cancer treatment. Nowadays, the trend of materials science has also contributed to the development of new nanoscale carrier systems, especially for use in the therapy of cancer patients. Many nanocarriers have been developed and continue to be developed for cancer therapy. Nanocarrier-based systems are widely used in cancer imaging, diagnostics, and therapeutics due to its promising properties and potential to increase therapeutic efficacy. The design and use of nano-based delivery systems for cancer therapy are very promising for the future of cancer therapy. This review article has been presented to the literature in order to display the prominent trends in cancer treatment in recent years. For this purpose, key points in designing an ideal nanoplatforms, the latest important nanocarriers such as liposomes, dendrimers, DNA origami, metallic nanoparticles, responsive, smart, biodegradable and biocompatible nanocarriers, together with recent studies, future perspectives are presented.Öğe α-Amylase Immobilized Composite Cryogels: Some Studies on Kinetic and Adsorption Factors(Springer, 2021) Acet, Omur; Inanan, Tulden; Acet, Burcu Onal; Dikici, Emrah; Odabasi, MehmetStability of enzymes is a significant factor for their industrial feasibility. alpha-Amylase is an important enzyme for some industries, i.e., textile, food, paper, and pharmaceutics. Pumice particles (PPa) are non-toxic, natural, and low-cost alternative adsorbents with high adsorption capacity. In this study, Cu2+ ions were attached to pumice particles (Cu2+-APPa). Then, Cu2+-APPa embedded composite cryogel was synthesized (Cu2+-APPaC) via polymerization of gel-forming agents at minus temperatures. Characterization studies of the Cu2+-APPaC cryogel column were performed by X-ray fluorescence spectrometry (XRF), scanning electron microscopy (SEM), and Brunauer, Emmett, Teller (BET) method. The experiments were carried out in a continuous column system. alpha-Amylase was adsorbed onto Cu2+-APPaC cryogel with maximum amount of 858.7 mg/g particles at pH 4.0. Effects of pH and temperature on the activity profiles of the free and the immobilized alpha-amylase were investigated, and results indicate that immobilization did not alter the optimum pH and temperature values. k(cat) value of the immobilized alpha-amylase is higher than that of the free alpha-amylase while K-M value increases by immobilization. Storage and operational stabilities of the free and the immobilized alpha-amylase were determined for 35 days and for 20 runs, respectively.
