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Öğe Digitalisation, bioinformatics, and delivery systems in sustainable nonthermal extraction of proteins(Oxford Univ Press, 2025) Jambrak, Anet Rezek; Nutrizio, Marinela; Dukic, Josipa; Djekic, Ilija; Vincekovic, Marko; Juric, Slaven; Pataro, GianpieroSustainable development integrates social, economic, technological, scientific, and environmental challenges through the framework of 17 Sustainable Development Goals. Industry 4.0, digitalisation, plant-based processing, environmental challenges, and alternative protein sources are vastly researched and present the foundation of innovative processing in the food processing industry. The exploitation of agroindustrial discards and plant-based by-products as an alternative source of valuable nutrients, including proteins, highlights the potential of upcycling (new economic value) while addressing sustainability challenges. Within the idea of nonthermal extraction of proteins and its process digitalisation, it is crucial to innovate, connect elements of Industry 4.0 and valorise the term sustainability. Innovative approaches in extractions such as ultrasound, plasma, pulsed electric fields, high-intensity pulses, and others, have an energy-saving effect with limited use of toxic chemicals and/or solvents. Usage of software for solvent selection, incorporating digitalisation, and development of protein and peptide databases can help in the prediction, optimisation, and efficiency of nonthermal extractions of proteins from agroindustrial discards. It is also important to select optimal delivery systems like electrospinning, electrospraying, or encapsulation as useful and efficient processes. The environmental, economic, and societal impact of innovative processing needs to be monitored and valorised using specific tools. Life cycle assessment methodology assesses the environmental impact throughout the life cycle of a commercial product, service, or process. The current review shows that extracting protein from agroindustrial discards and by-products of plant and animal origins is a complex matter. The need for a comprehensive approach that considers various viewpoints on extraction is suggested.Öğe Identification and Assessment of Therapeutic Phytoconstituents of Catharanthus roseus through GC-MS Analysis(Mdpi, 2023) Rani, Jyoti; Kapoor, Manish; Dhull, Sanju Bala; Goksen, Gulden; Juric, SlavenThe leaves of Catharanthus roseus (L.) G. Don contain a large number of diverse secondary metabolites, making them comparably complex. The Catharanthus genus has received increased interest from scientists in recent years due to its extensive applications in several domains, including the pharmaceutical sector, where precise characterization of its characteristics is required. An effective inquiry technique is needed for chemo-profiling to identify the metabolites in plant samples. The main goal of this research is to provide supplementary data on the chemical composition of the leaves of twenty-five different accessions of C. roseus through the application of gas chromatography-mass spectrometry (GC-MS). The study's findings reveal the existence of a vast number of phytochemicals, allowing for a comparison of the different accessions. Furthermore, a meticulous statistical analysis of this data using principal components analysis (PCA) and a heatmap, and hierarchical cluster analysis (HCA) may aid in providing more relevant information on C. roseus leaves for possible investigation of their metabolites in further scientific studies.Öğe Innovative Applications of Electrospun Nanofibers Loaded with Bacterial Cells Towards Sustainable Agri-Food Systems and Regulatory Compliance(Springer, 2024) Juric, Marina; Goksen, Gulden; Donsi, Francesco; Juric, SlavenThis comprehensive review highlights the innovative applications of eco-friendly nanofibers in facilitating microbial activity and functionality for the advancement of sustainable agri-food systems. It provides an in-depth exploration of nanofiber-based biocomposites, with a specific focus on the encapsulation of bacterial cells within nanofibers. The review emphasizes the pivotal role of advanced 3D nanostructured scaffolds, appropriately designed for the encapsulation and survivability of these bacterial cells in response to specific environmental triggers. Furthermore, this paper delves into the utilization of nanofibers as nanocarriers to enhance the functionality of bacteria. It illustrates the need for comprehensive safety assessments, especially considering the potential risks involved. The assessment establishes a connection between the technical discussions and the environmental regulations that support the implementation of sustainable practices within the agri-food sector. The review highlights the vital role played by international guidelines, such as those established by the United Nations Environment Program (UNEP) and the Food and Agriculture Organization (FAO), in guiding the formulation of national and regional policies. By interconnecting these strategic principles with technological advancements in nanofiber technology, the review favors the incorporation of green, eco-friendly nanofiber production via an electrospinning method to revolutionize the agri-food sector. This holistic approach aims to address current challenges, paving the way for sustainable progress.