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    Digitalisation, bioinformatics, and delivery systems in sustainable nonthermal extraction of proteins
    (Oxford Univ Press, 2025) Jambrak, Anet Rezek; Nutrizio, Marinela; Dukic, Josipa; Djekic, Ilija; Vincekovic, Marko; Juric, Slaven; Pataro, Gianpiero
    Sustainable development integrates social, economic, technological, scientific, and environmental challenges through the framework of 17 Sustainable Development Goals. Industry 4.0, digitalisation, plant-based processing, environmental challenges, and alternative protein sources are vastly researched and present the foundation of innovative processing in the food processing industry. The exploitation of agroindustrial discards and plant-based by-products as an alternative source of valuable nutrients, including proteins, highlights the potential of upcycling (new economic value) while addressing sustainability challenges. Within the idea of nonthermal extraction of proteins and its process digitalisation, it is crucial to innovate, connect elements of Industry 4.0 and valorise the term sustainability. Innovative approaches in extractions such as ultrasound, plasma, pulsed electric fields, high-intensity pulses, and others, have an energy-saving effect with limited use of toxic chemicals and/or solvents. Usage of software for solvent selection, incorporating digitalisation, and development of protein and peptide databases can help in the prediction, optimisation, and efficiency of nonthermal extractions of proteins from agroindustrial discards. It is also important to select optimal delivery systems like electrospinning, electrospraying, or encapsulation as useful and efficient processes. The environmental, economic, and societal impact of innovative processing needs to be monitored and valorised using specific tools. Life cycle assessment methodology assesses the environmental impact throughout the life cycle of a commercial product, service, or process. The current review shows that extracting protein from agroindustrial discards and by-products of plant and animal origins is a complex matter. The need for a comprehensive approach that considers various viewpoints on extraction is suggested.
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    Probing the impact of sustainable emerging sonication and DBD plasma technologies on the quality of wheat sprouts juice
    (Elsevier, 2023) Manzoor, Muhammad Faisal; Hussain, Abid; Goksen, Gulden; Ali, Murtaza; Khalil, Anees Ahmed; Zeng, Xin-An; Jambrak, Anet Rezek
    Sonication and dielectric barrier discharge (DBD) plasma are sustainable emerging food processing technologies. The study investigates the impact of sonication, DBD-plasma, and thermal treatment (TT) on wheat sprout juice. The obtained results indicated a significant (p < 0.05) increase in chlorophyll, total phenolics, flavonoids, DPPH assay, and ORAC assay after DBD-plasma (40 V) and sonication (30 mins) treatment as compared to TT and untreated samples. Both emerging technologies significantly (p < 0.05) reduce the polyphenol oxidase and peroxidase activities, but the TT sample had the highest reduction. Moreover, the synergistic application of both technologies significantly reduced the E. coli/Coliform, aerobics, yeast and mold up to the 2 log reduction, but the TT sample had a complete reduction. DBD-plasma and sonication processing significantly decreased (p < 0.05) the particle size, reducing apparent viscosity (eta) and consistency index (K); while increasing the flow behavior (n), leading to higher stability of wheat sprout juice. To assess the impact of emerging techniques on nutrient concentration, we used surface-enhance Raman spectroscopy (SERS) as an emerging method. Silver -coated gold nano-substrates were used to compare the nutritional concentration of wheat sprout juice treated with sonication, DBD-plasma, and TT-treated samples. Results showed sharp peaks for samples treated with DBD-plasma followed by sonication, untreated, and TT. The obtained results, improved quality of wheat sprout juice, and lower microbial and enzymatic loads were confirmed, showing the suitability of these sustainable processing techniques for food processing and further research.