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    Association of Kruppel-like factor 2 polymorphisms with the risk of coronary artery disease
    (Cukurova Univ, Fac Medicine, 2023) Degirmenci, Ulas; Yildirim, Metin; Akkapulu, Merih; Yalin, Ali Erdinc; Yilmaz, Dilek Cicek; Oztornaci, Ragip Onur; Tasdelen, Bahar
    Purpose: Coronary artery disease is defined as complications that develop in proportion to the prevalence of ischemia due to occlusion of the coronary arteries and the resulting cell death. The development of atherosclerosis is significantly influenced by endothelial cell dysfunction. Kruppel-like factor 2, a transcription factor, has been shown to regulate critical biological events in endothelial biology, such as vascular tone, migration, proliferation, vasoreactivity, and angiogenesis. In our study, it was aimed to clarify the relationship between coronary artery disease and Kruppel-like factor 2 protein levels and C1239A polymorphisms.Materials and Methods: 191 individuals who underwent coronary angiography at Mersin University Faculty of Medicine, Department of Cardiology, Health, Research and Application Center were included in the study. Measurements of serum fasting blood glucose, HDL cholesterol, total cholesterol, and triglyceride levels were performed on the AU5800 (Beckman Coulter, United States) autoanalyzer. Serum LDL levels were calculated using the Friedwald equation. Serum Kruppel-like factor 2 protein levels were measured by sandwich enzyme-linked immunoassay method on the Multiskan GO (Thermo Scientific, Finland) device. Kruppel-like factor 2 C1239A variations were detected on the Applied Biosystem VIIATM 7 Real-Time PCR (Life Technologies Co., United States) device by TaqMan (R) single nucleotide polymorphism (SNP) genotyping method.Results: Men had a 3.8-fold higher risk of CAD than women. (Odd's ratio 3.83, 95% Confidence interval 1.98- 7.39; p<0.001). Kruppel-Like Factor 2 protein levels werenot significantly different amongst the groups. Kruppel-like factor 2 C1239A polymorphisms were not associated with coronary artery disease.Conclusion: More comprehensive studies with larger sample groups including other gene regions and meta -analysis studies are needed to determine the relationship between Kruppel-like factor 2 gene polymorphisms and coronary artery disease.
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    Possible protective role of punicalagin on oxidative stress, inflammation and genotoxicity in ethanol-induced liver toxicity
    (Marmara Univ, 2021) Yanpar, Esma; Yildirim, Metin; Akkapulu, Merih; Degirmenci, Ulas; Konen Adiguzel, Serpil; Yalin, Serap; Yalin, Ali Erdinc
    Alcohol consumption is a tradition in most cultures but are increasingly being arised as possessing a potential for misuse. Punicalagin is a phenolic compound that is found in forms alpha and beta in pomegranates. In this study, the protective role of punicalagin on oxidative stress, inflammation and DNA damage caused by ethanol (EtOH) in liver tissue were examined. Wistar albino rats were divided into 4 groups as control, eth, punicalagin, EtOH EtOH + punicalagin. In EtOH groups, rats were treated with EtOH (4g/kg) for 21 days, in punicalagin groups, rats were treated with punicalagin (4 mg/ kg) for 21 days. In the liver tissue, superoxide dismutase (SOD), catalase (CAT) activities and malondialdehyde (MDA) and glutathione (GSH) levels, in serum AST, ALT, LDH activities and TNF-alpha, IL-6 levels were measured. Genotoxicity was evaluated using comet assay. Based on these experimental results, while EtOH increased ALT, AST and LDH enzyme activies and induced inflammation and oxidative stress. Punicalagin reduced IL6, TNF-alpha, MDA levels, ALT, AST, LDH enzyme activities and increased SOD, CAT activities and GSH levels. EtOH significantly increased the percentage of damaged cells (type II, III and IV) and genetic damage index compared to the other groups (control, punicalagin and EtOH +punicalagin). Punicalagin was not genotoxic compared to the control. Furthermore, punicalagin reduced the genotoxic effect, induced by EtOH, with the sharp decrease in damaged cells (from 14.00 +/- 1.22 to 2.20 +/- 1.30) and genetic damage index (from 1.20 +/- 0.05 to 0.14 +/- 0.05). Punicalagin has antioxidant, anti-inflammatory and protective role against to ethanol induced liver toxicity.
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    Öğe
    Potential Protective Effects of Ruta Chalepensis L. Extracts Against Gentamicin-Induced Nephrotoxicity via Reduction on Apoptotic, DNA Damage and Oxidative Stress Markers in Mice
    (Taylor & Francis Ltd, 2023) Yildirim, Metin; Ersatir, Mehmet; Degirmenci, Ulas; Yigin, Akin; Unal, Mustafa Boyraz; Guldur, Muhammet Emin; Demirkol, Onur
    In this study, the protective effects of Ruta chalepensis L. extracts on the extent of tissue damage in gentamicin-induced nephrotoxicity have been investigated. Ruta chalepensis L. extracts were prepared by subcritical water and ultrasound-assisted organic solvent extraction methods. Protective activity of Ruta chalepensis L. extracts on Gentamicin-induced nephrotoxicity is investigated by apoptotic, DNA damage, oxidative stress markers and evaluating histopathological in kidney tissue of mice. Gentamicin significantly increased Caspase-3 and -8 activities, NO levels, serum creatinine and BUN, while 8-OHdG and MDA levels were significantly decreased with Ruta chalepensis L. extract treatment. In addition, Ruta chalepensis L. extracts treatment significantly increased CAT and SOD activities. Histopathological alterations in Gentamicin group were significantly diminished by application of Ruta chalepensis L. extracts. These results suggest that treatment with Ruta chalepensis L. extracts may ameliorate renal dysfunction and structural damage through the reduction of oxidative stress and apoptosis in the kidney.