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    Evaluation of photodynamic therapy and nuclear imaging potential of subphthalocyanine integrated TiO2 nanoparticles in mammary and cervical tumor cells
    (World Sci Publ Co Inc, 2019) Yurt, Fatma; Ocakoglu, Kasim; Er, Ozge; Soylu, Hale Melis; Ince, Mine; Avci, Cigir Biray; Kurt, Cansu Caliskan
    This study, subphthalocyanines (SubPc) and SubPc integrated TiO2 nanoparticles (SubPc-TiO2) were synthesized as novel photosensitizers. Their PDT effects were evaluated. Furthermore, nuclear imaging potential of I-131-labelled SubPc/SubPc-TiO2 were examined in mouse mammary carcinoma (EMT6) and cervix adenocarcinoma (HeLa) cell lines. The uptake results show that SubPc labelled with I-131 radionuclide ((131) I-SubPc) in EMT6 and HeLa cell lines was found to be approximately the same as in the WI38 cell line. However, the uptake values of SubPc-TiO2 labelled with I-131(I-131-SubPc- TiO2) in EMT6 and HeLa cell lines were determined to be two times higher than in the WI38 cell line. In other words, the target/non-target tissue ratio was identified as two in the EMT6 and HeLa cell lines. I-131-SubPc-TiO2 is promising for imaging or treatment of breast and cervix tumors. In vitro photodynamic therapy studies have shown that SubPc and SubPc-TiO2 are suitable agents for PDT. In addition, SubPc-TiO2 has higher phototoxicity than SubPc. As a future study, in vivo experiments will be held and performed in tumor-bearing nude mice.
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    Photodynamic therapy potential of cobalt phthalocyanine in triple-negative breast cancer
    (Pergamon-Elsevier Science Ltd, 2023) Dogan, Sifa; Ince, Mine; Sogutlu, Fatma; Avci, Cigir Biray; Ozel, Derya; Yurt, Fatma
    Triple-negative breast cancer (TNBC) has the worst prognosis among breast cancers. The deficiency of hormone receptors (ER-/PR-/HER-2-) limits treatment options. Photodynamic therapy (PDT) is a safe treatment method for target-specific applications and has no toxic side effects. In this study, the efficacy of PDT on TNBC cells after activation of phthalocyanine derivative CoPc-COOH with RED light was investigated. The cytotoxicity of CoPc-COOH in MDA-MB-231 cells was evaluated at 24, 48, and 72 h. To detect phototoxic activity, cells were exposed simultaneously to a dose range of CoPc-COOH at 48th hours and 20, 30, 40, and 50 J/cm2 RED light in a laser source with an output fluorescence of 12 mW/cm2. The apoptotic effect of single CoPc-COOH, RED light, and their combinations were examined by using the Annexin V method and the ROS generation potential using the carboxy-H2-DCFH-DA test in flow cytometry. DCF fluorescence intensity of control and dose groups were taken under the fluorescence microscope. The IC50 values of CoPc-COOH in MDA-MB-231 cells at the 24th, 48th, and 72nd hours were calculated as 131.04 mu M, 66.75 mu M, and 59.74 mu M, respectively. In the phototoxicity test, 50 J/ cm2 application increased the IC50 dose of CoPc-COOH to 73.73 mu M; 20, 30, 40 J/cm2 application reduced the doses to 2.31 mu M, 0.20 mu M, and 28.14 mu M, respectively. Compared to the untreated group, it was determined that ROS formation and apoptotic stimulation were triggered significantly in treatment groups. A combination of CoPc-COOH and 40 J/cm2 showed the highest synergistic effect.